采油微生物YM4中试发酵培养基配方优化  

Optimization of Formula of Medium for Fermentation by YM4 of Oil Recovery Microorganism

作　　者：薛媛 康宵瑜 王蓓蕾 

机构地区：[1]陕西延长石油(集团)有限责任公司研究院,陕西西安710075

出　　处：《广东化工》2014年第21期45-46,56,共3页Guangdong Chemical Industry

基　　金：陕西省科技统筹创新工程计划项目

摘　　要：对一株采油铜绿假单胞菌YM4发酵生产鼠李糖脂的培养基组成及培养条件进行了优化,优化后培养基组成为:糖蜜粉4%,玉米浆0.5%(V/V);最佳培养条件为:500 m L三角瓶装液量为100 m L,接种量10%,初始p H 7.2,35℃,180 r/min摇床培养24 h。在此条件下,发酵液的表面张力可达到28.08 m N/m,界面张力达到10-2 m N/m,比优化前节约原料成本74%。The effects of fermentation conditions on rhamnolipid produced by Pseudomonas aeruginosa were investigated through the single factor experiments. The optimized compositions of the medium were： Molasses 4 %, CSL 0.5 %（V/V）; The optimum conditions were：100ml in 500 m L total volume, seed volume 10 %（V/V）, the temperature of the shaking table 35 ℃, rotating speed 180 r/min, the initial p H 7.2, the culture time 24 h. Under the optimum conditions, the surface tension of fermentation is 28.08 m N/m, the interfacial tension of fermentation is 10-2 m N/m, the cost of material was decreased by 74 %.

关 键 词：铜绿假单胞菌 鼠李糖脂 表面张力 界面张力 发酵 

分 类 号：TQ423[化学工程]