紫杉醇联合5-Aza-dC对非小细胞肺癌细胞NCI-H446生长抑制作用的研究  

Inhibitory effect of Paclitaxel combined with 5-Aza-dC on non-small cell lung cancer cell line NCI-H446

作　　者：曲红梅 姜艳辉 孙伟 朱新冰 

机构地区：[1]辽河油田总医院急诊科,辽宁盘锦124010 [2]辽河油田总医院普外科,辽宁盘锦124010

出　　处：《海南医学》2014年第21期3130-3133,共4页Hainan Medical Journal

摘　　要：目的探讨紫杉醇(PAC)联合DNA甲基化酶抑制剂5-氮杂-2'-脱氧胞苷(5-Aza-d C)对非小细胞肺癌细胞NCI-H446增殖迁徙及侵袭能力的影响。方法将人小细胞肺癌细胞NCI-H446给予紫杉醇及5-Aza-d C干预处理,根据药物干预方式的不同分为对照组、紫杉醇组及联合用药组;应用CCK-8法检测各组NCI-H446细胞增殖能力的差异;划痕实验和Transwell实验检测各组NCI-H446细胞迁移和侵袭能力的差异。结果 CCK-8结果显示PAC及5-Aza-d C均可抑制NCI-H446细胞的增殖活性,且呈时间依赖性。48 h后紫杉醇组及联合用药组NCI-H446细胞的增殖能力较对照组明显减弱(P<0.01);划痕实验结果显示划痕形成24 h后,对照组划痕愈合率为(69.15±0.837)%。而在紫杉醇组划痕处愈合率为(47.30±0.783)%;联合用药组划痕愈合率为(43.45±0.862)%。紫杉醇组及联合用药组NCI-H446细胞在迁移能力方面较对照组差异有统计学意义(P<0.01);Transwell实验结果显示对照组NCI-H446细胞转染穿过基质胶的细胞数量为(151.4±6.88)个,紫杉醇组为(66.8±5.17)个,联合用药组为(40.2±4.55)个。紫杉醇组及联合用药组NCI-H446细胞的侵袭能力较对照组差异有统计学意义(P<0.01)。结论 5-Aza-d C作为化疗增敏剂可增加非小细胞肺癌细胞NCI-H446对紫杉醇的敏感性,从而提高紫杉醇对非小细胞肺癌的化疗效果。Objective To investigate the effect of Paclitaxel （PAC） combined with DNA methyltransferase inhibitor 5-aza-2＇-deoxycytidine （5-Aza-dC） on the proliferation, invasion and migration of on non-small cell lung cancer （NSCLC） cell line NCI-H446. Methods NSCLC cell lines NCI-H446 were treated with PAC and 5-Aza-dC, and divided into three groups： control group, PAC group and combined group. NCI-H446 cell＇s proliferation ability was analyzed by CCK-8 assay, while the migration and invasion abilities were assessed by wound-healing assay and Matrigel invasion assay. Results CCK-8 assay indicated that NCI-H446 cells treated with PAC and 5-Aza-dC exhib- ited growth retardation. 48 h after treatment, the proliferation ability of PAC group and combined group was signifi- cantly reduced compared with that of the control group （P〈0.01）. 24 h after wound formation, the wound healing rate of control group was （69.15±0.837）%, compared with （47.30±0.783）% in the PAC group and only （43.45±0.862）% in the combined group. These results showed cell migration in PAC group and combined group was significantly re- duced, compared with the control group （P〈0.01）. Matfigel invasion assay showed that the numbers of cells penetrat- ing Matrigel and adhering to the membrane in the control, PAC, and combined groups was （151.4±6.88）, （66.8±5.17）, （40.2±4.55）, respectively, which indicated that cell invasion in PAC, and combined groups were significantly reduced （P〈0.01）. Conclusion 5-Aza-dC can enhance the chemosensitivity of NSCLC cell line NCI-H446 to PAC, and thus improve the chemotherapeutic effect of PAC for NSCLC.

关 键 词：非小细胞肺癌 5-Aza-d C 紫杉醇 化疗增敏剂 

分 类 号：R734.2[医药卫生—肿瘤]