3,5-二硝基水杨酸法测定枸杞提取液中多糖的含量  

Determination of Polysaccharide Content in Extraction Solution of Lycrium Barbarum with 3,5-Dinitrosalicylic Acid Method

作　　者：樊晓辉[1] 赵春玲[1] 张成军[1] 李阳[1] 万端极[1] 

机构地区：[1]湖北工业大学轻工学部,湖北武汉430068

出　　处：《食品研究与开发》2014年第21期77-80,共4页Food Research and Development

基　　金：湖北省省级大学生创新创业训练计划(201310500019)

摘　　要：建立3,5-二硝基水杨酸(DNS)法测定枸杞提取液中多糖含量的方法。结果表明:测定波长为540 nm,显色剂用量为3.0 m L,沸水浴时间为5 min,稳定时间40 min以内。方法的线性、精密度和加标回收率均较为理想。该方法准确度高,且比苯酚-硫酸法更易于操作,适合工业化生产控制。3,5-dinitrosalicylic acid （DNS） method was used to determine the content of polysaccharide in extraction solution of Lycium barbarum. The results showed that detection wavelength was 540 nm , the dosage of DNS solution was 3.0 mL, the boiling bath time was 5 min and the stable time was 40 min. The proposed method had good liner relationship, precision and average recovery rate. The method with high accuracy was easier to operate than phenol-sulfuric acid method,and it especially was suitable for determining polysaccharide in the industrial process.

关 键 词：枸杞 多糖 3 5-二硝基水杨酸法 提取液 

分 类 号：TS201.2[轻工技术与工程—食品科学]