百蕊草的吸器诱导及发育信号物质的分离鉴定  

Identification of chemical signals and haustorium induced of Thesium Chinense

作　　者：刘波[1] 张晓明[1] 郭巧生[1] 王长林[1] 陈璐[1] 宋玲珊[1] 安开龙 

机构地区：[1]南京农业大学中药材研究所,江苏南京210095

出　　处：《中国中药杂志》2014年第23期4544-4547,共4页China Journal of Chinese Materia Medica

基　　金：国家自然科学基金项目(81173482)

摘　　要：利用GC-MS检测鉴定百蕊草优势寄主植物夏枯草根系分泌物,并对2,6-二甲氧基-对苯醌(DMBQ)对百蕊草吸器诱导作用进行了初步研究。通过分析鉴定得到了9类53种化合物,其中大多数为烃类化合物、酯类化合物和有机酸类化合物,而酚酸类、醛类和醌类化合物种类则较少,分别只检测出1种。其中2,5-二叔丁基-1,4-苯醌具有1,4-对苯醌核心结构,可能对夏枯草与百蕊草寄生关系的建立具有重要意义。DMBQ能够诱导百蕊草根系产生吸器,并且1μmol·L-1DMBQ对百蕊草吸器的诱导能力最强。To separate and identify chemical signals which induce Thesium chinense haustorium formation, the components of T. chinense roots secretion collected with XAD-4 resin were detected by GC-MS. The effect of DMBQ as exogenous signals to induce haustorium formation in T. chinense was studied. Fifty-three compounds of 9 types had been detected, including hydrocarbons, esters, organic acids, ketones, alcohols, nitrogen containing compounds, phenolic acids, aldehyde and quinine. It is worth noting that the 2, 5-di-tert-butyl-1,4-benzoquinone has the core structure of 1,4-benzoquinone, which may play an important role in the parasitic relationship of Prunella vulgaris and T. chinense. DMBQ worked effectively on inducing haustoria, but induction effects vary widely in different concentrations. DMBQ with the concentration of 1 μmol·L^-1 showed the best effect of the inducing ability with a ratio of 110.52 when treated to induce haustoria.

关 键 词：百蕊草 吸器 气质联用 信号物质 

分 类 号：R284.2[医药卫生—中药学]