香樟木质部挥发性成分的SPME-GC/MS分析  

Analysis of Volatile Components in Cinnamomum camphora Xylem by SPME-GC/MS

作　　者：李权[1] 王晓娴[1] 林金国[1] 江茂生[2] 初雷霞[2] 

机构地区：[1]福建农林大学材料工程学院,福建福州350002 [2]福建农林大学生命科学学院,福建福州350002

出　　处：《质谱学报》2014年第6期555-562,共8页Journal of Chinese Mass Spectrometry Society

基　　金：国家级星火计划重大项目(2012GA720001);国家林业局林业公益性行业科研专项项目(200704010)资助

摘　　要：采用固相微萃取技术(SPME)吸附香樟木质部挥发性成分,结合气相色谱-质谱(GC/MS)进行化学成分的分离和鉴定,同时比较不同色谱柱对分离效果的影响。结果表明:采用弱极性DB-5MS色谱柱共分离出51个组分,解析出43种挥发性成分,占总峰面积的97.04%,分离效果和所得的峰形较好于DB-WAXetr色谱柱。鉴定出含量较高的成分有:左旋-α-蒎烯(4.57%)、莰烯(2.14%),双环[3.1.0]-4-甲基-1-异丙基-2-己烯(4.16%)、β-蒎烯(2.3%)、邻异丙基甲苯(2.15%)、D-柠檬烯(7.49%)、桉叶油醇(13.85%)、樟脑(38.71%)、(R)-4-萜品醇(1.74%)、α-松油醇(2.40%)、黄樟素(2.96%)、α-荜澄茄油烯(4.36%)、1-石竹烯(1.92%)等。该方法可为香樟材在医药、食品和化学工业等方面的进一步开发和利用提供参考。The volatile components of Cinnamomum camphora xylem were extracted by solid phase microextraction（SPME） and were identified by gas chromatography-mass spectrometry （GC/MS）.The separation efficiency of different chromatographic column was compared as well.The results show that GC/MS spectrometric analysis with DB-5MS isolates 51 components,which contains 43 kinds of volatile content,and the proportion of the peak area of volatile components is 97.09 ％.The separation efficiency and the peak shape of GC/MS spectrometric analysis with DB-5MS are superior to that with DB-WAXetr.Based on the identification of the volatile components,（1S）-α-pinene（4.57％）,camphene（2.14％）,bicyclo[3.1.0]-4-methyl-l-（1-methylethyl）-hex-2-ene（4.16％）,β-pinene（2.3％）,1-methyl-2-（1-methylethyl）-benzene（2.15 ％）,D-limonene （7.49 ％）,eucalyptol （13.85 ％）,camphor（38.71％）,（R）-4-terpineol（1.74％）,α-terpineol（2.40％）,safrole（2.96％）,α-cubebene （4.36％） and caryophyllene（1.92％） account for most of them.The method can provide a reference for the further development and utilization of Cinnamomum camphora in medicine,food and chemical industry,etc.

关 键 词：香樟 固相微萃取 挥发性成分 气相色谱-质谱(GC/MS) 

分 类 号：O657.63[理学—分析化学]