遗传性癫痫大鼠海马NOs、SOD、MDA、LDH的异常变化  

The abnormal changes of NOs, SOD, MDA and LDH in the hippocampus of genetic epilepsy Wistar rats

作　　者：王千慧[1] 徐晓雪[2] 赵丹[3] 高青华[1] 朱茂华[1] 张娟[1] 封瑞[1] 马中女[1] 蔡际群[1] 郝丽英[1] 郭凤[1] 

机构地区：[1]中国医科大学药学院药物毒理教研室,辽宁沈阳110001 [2]中国医科大学附属一院神经内科,辽宁沈阳110001 [3]陕西省榆林市第二医院,陕西榆林719000

出　　处：《解剖科学进展》2014年第6期559-561,566,共4页Progress of Anatomical Sciences

基　　金：国家自然科学基金资助项目(No.81001429)

摘　　要：目的比较遗传性癫痫大鼠(tremor,TRM)海马与正常Wistar大鼠海马的一氧化氮合酶(NOs)、超氧化物歧化酶(SOD)、丙二醛(MDA)、乳酸脱氢酶(LDH)的变化,进一步揭示癫痫的发病机制。方法应用Western Blot法检测TRM海马区NOs蛋白的表达;应用试剂盒检测TRM海马区LDH和SOD的活性以及MDA的含量。结果与正常Wistar大鼠相比,遗传性癫痫大鼠海马中NOs表达水平升高,MDA含量和LDH活性升高(<0.01),SOD活性无变化。结论 NOs、LDH、MDA的异常变化可能与遗传性癫痫大鼠的癫痫发生与发展相关。Objective To compare the expressions of Nitric oxide synthase（NOs）, superoxide dismutase（SOD）, malondialdehyde（MDA） and lactate dehydrogenase（LDH） in hippocampus of genetic epilepsy rats（tremor, TRM） with normal Wistar rats to further explore the pathogenesis of epilepsy. Methods The expression of NOs protein in hippocampus was detected by Western Blot method, the activities of SOD and LDH and the content of MDA in hippocampus were detected by corresponding commercial kits. Results The expression level of NOs protein was significantly elevated, the content of MDA was increased, the activity of LDH was augmented in TRM than in control rats（P〈0.05）, but without significant difference for SOD between TRM and control rats（P〉0.05）. Conclusion Abnormal changes of NOs, LDH and MDA may be related to the pathogenesis of TRM.

关 键 词：遗传性癫痫大鼠 海马 一氧化氮合酶 超氧化物歧化酶 丙二醛 乳酸脱氢酶 

分 类 号：R742.1[医药卫生—神经病学与精神病学]