黄芪减轻血管紧张素Ⅱ诱导的小鼠血管重塑  

Astragalus membranaceus alleviates angiotensin Ⅱ induced vascular remodeling in mice

作　　者：裴芳[1,2] 王新全[2] 岳荣川[2] 黄骥[1] 黄婕[1] 王旭开[2] 于长青[2] 

机构地区：[1]中国人民武装警察部队重庆市总队医院心血管内科,重庆400061 [2]第三军医大学大坪医院野战外科研究所心血管内科重庆市心血管病研究所,重庆400042

出　　处：《基础医学与临床》2014年第12期1606-1610,共5页Basic and Clinical Medicine

基　　金：国家杰出青年科学基金(30925018);国家自然科学基金(81200193)

摘　　要：目的探讨黄芪对血管紧张素Ⅱ(AngⅡ)诱导的血管重塑的作用及其可能机制。方法将小鼠随机分为对照组、AngⅡ组、AngⅡ+氯沙坦治疗组及AngⅡ+黄芪治疗组,每组各10只。对照组给予0.9%氯化钠注射液0.2 m L/d灌胃,其余3组均皮下埋微渗透泵,以200 ng/(kg·min)的剂量缓慢释放AngⅡ建立血管重塑模型。其中氯沙坦治疗组以10 mg/(kg·d)剂量灌胃,黄芪治疗组以20 g/(kg·d)剂量灌胃。所有小鼠总计处理14 d。每2天以尾套法测定收缩压。第14天时处死小鼠,收集主动脉进行HE及Masson染色检测血管重塑情况。RT-PCR检测主动脉Ⅰ型胶原蛋白转录水平。Western blot检测血管紧张素转换酶2(ACE2)及AngⅡ1型受体(AT1R)蛋白表达。结果黄芪处理可以显著降低AngⅡ引起的血压升高(P<0.05)、减轻AngⅡ引起的主动脉管壁增厚和纤维化增加,降低Ⅰ型胶原蛋白mRNA表达(P<0.05),上调ACE2蛋白表达(P<0.05)并下调AT1R蛋白表达(P<0.05)。结论黄芪可减轻AngⅡ诱导的血管重塑,其机制可能是通过上调ACE2和下调AT1R发挥作用。Abstract： Objective To investigate the vascular remodeling in mice and its unde effect of astragalus dying mechanism. membranaceus on angiotensin Ⅱ （AngⅡ ） induced Methods Mice were randomly divided into 4 groups including control group, AngⅡ group, AngⅡ ＋ Losartan group and AngⅡ ＋ Astragalus group. In control group, mice were given 0. 2 mL/d normal saline by gastric gavage. In the other 3 groups, mice were given 200 ng/（kg · min）AngⅡ by an osmotic pump embedded subcutaneously. In addition, in AngⅡ ＋ Losartan group, mice were given 10 mg/（ kg · d） Losartan by gastric gavage; in Ang Ⅱ＋ Astragalus group, mice were given 20 g/（ kg · d） by gastric gavage. All the mice were treated for 14 days. Systolic pressure was measured by the tail-cuff method every 2 days. Mice were sacrificed on day 14 and aortas were collected. HE and Masson＇s staining were performed to observe vascular remodeling. RT-PCR was carried out to detect transcriptional expression of collagen Ⅰ. Western blot was performed to detect expression of angiotensin converting enzyme 2 （ACE2） and angiotensin Ⅱtype 1 receptor （AT1 R）. Results Administration of astragalus membranaceus significantly decreased Ang Ⅱ induced blood pressure elevation （P 〈 0.05）, attenuated thickening and fibrosis of aorta, decreased mRNA level of collagenⅠ （P〈0.05）, up Conclusions Astragal -regulated ACE2 expression （ P 〈 0. 05） and down-regulated AT1 R expression （P 〈 0. 05）. us membranaceus alleviates vascular remodeling induced by Ang II in mice, possibly through up-regulating expression of ACE2 and down-regulating AT1 R.

关 键 词：血管紧张素Ⅱ 血管重塑 纤维化 黄芪 ANGIOTENSIN Ⅱ 

分 类 号：R285.5[医药卫生—中药学]