干扰素α通过P204和P21诱导大鼠原代血管平滑肌细胞凋亡  

Interferon alpha induces apoptosis of rat primary vascular smooth muscle cells via P204 and P21

作　　者：龙向淑[1] 吴强[1] 宋方[1] 黄晶[1] 张林军[1] 

机构地区：[1]贵州省人民医院心血管内科,贵州贵阳550002

出　　处：《基础医学与临床》2014年第12期1635-1639,共5页Basic and Clinical Medicine

基　　金：国家自然科学基金(81260030);贵州省优秀科技教育人才省长专项资金[黔省专合字(2009)30号]

摘　　要：目的探讨干扰素-α(IFN-α)对大鼠血管平滑肌细胞(VSMCs)凋亡的影响。方法应用转染IFI204 siRNA和/或IFN-α瞬时干预体外培养的大鼠VSMCs,以非特异性siRNA转染组为对照组,RT-PCR法检测P204及P21mRNA表达,Western blot检测P204及P21蛋白表达,流式细胞仪Annexin-V FITC/PI法检测细胞凋亡。结果与对照组比较,IFN-α组P204 mRNA和蛋白表达上调(P<0.01),细胞凋亡增多(P<0.01),伴P21 mRNA和蛋白表达增多(P<0.01);IFI204 siRNA转染组P204 mRNA和蛋白表达下调(P<0.01),细胞凋亡减少(P<0.01),P21mRNA和蛋白表达减少(P<0.01);转染IFI204 siRNA后再加IFN-α干预,P204 mRNA及蛋白表达下调(P<0.01),但P21mRNA及蛋白表达增多(P<0.01),促进细胞凋亡(P<0.01)。结论 P204及P21参与IFN-α诱导大鼠VSMCs凋亡过程的调控。Objective To study the effect of interferon alpha （IFN-ot） on vascular smooth muscle cells （VSMCs） apoptosis in rats. Methods Cultured VSMCs were treated with transfection IFI204 siRNA and/or IFN-otin vitro instantaneously. Nonspecific siRNA transfection group was as control group. The expression of P204 and P21 mRNA was determined by semiquantitative RT-PCR. P204 and P21 protein was analyzed by Western blotting. The cell apoptosis was analyzed by flow cytometry with Annexin-V FITC/PI method. Results Compared with control group, in IFN-αinduction group, the expression of P204 mRNA and protein was up-regulated （P 〈 0.01 ） , the cell apoptosis increased （P 〈0.01 ） , and the expression of P21 mRNA and protein was up-regulated （P 〈 0.01 ）. In IFI204 transfection group, the expression of P204 mRNA and protein was down-regulated（ P 〈 0.01 ）, the cell apoptosis decreased （P 〈0.01 ） , and the expression of P21 mRNA and protein was down-regulated （P 〈 0.01 ）. When intervention with IFN-α after transfection IFI204 siRNA, the expression of P204 mRNA and protein was down-regulated （P 〈 0.01 ） , but the expression of P21 mRNA and protein were not decreased correspondingly （P 〈 0. 01 ）, and cell apoptosis increased （ P 〈 0.01 ）. Conclusions P204 and P21 participation in interferon-or regulation of cell apoptosis of vascular smooth muscle cells in rats.

关 键 词：干扰素 血管平滑肌细胞 凋亡 干扰素诱导蛋白 P204 

分 类 号：R363[医药卫生—病理学]