肝癌组织中KLF17的表达及临床意义  

KLF17 expression and clinical significance in hepatocellular carcinoma

作　　者：孙昭[1] 赵林[1] 周娜[1] 高鹤丽[1] 马春亮[1] 韩钦[2] 

机构地区：[1]中国医学科学院北京协和医院肿瘤内科,北京100730 [2]中国医学科学院基础医学研究所组织工程研究中心,北京100005

出　　处：《基础医学与临床》2014年第12期1640-1644,共5页Basic and Clinical Medicine

基　　金：国家自然科学基金(81101588;30800429)

摘　　要：目的 检测KLF17在肝癌组织的表达并评价其对肝癌预后的影响.方法 免疫组化法检测KLF17在肿瘤组织中的表达,以及KLF17表达对患者无进展生存和总生存期的影响.比较原代培养的Hep11和Hep12细胞侵袭和迁移的差异,以及KLF表达的差异.干扰KLF17表达后Transwell法检测Hep11迁移和侵袭的能力.结果 36名有随访结果并可评估的患者中,KLF17 0 ～2分19人,3～5分的17人.KLF17 3～5分的患者无进展生存期较0～2分的患者长,有统计学差异,分别为（39.3±4.2）个月和（23.4±4.9）个月（P＜0.05）.KLF17 3～5分的患者总生存为（46.9±3.2）个月,KLF17 0 ～2分的患者为（35.2±4.2）个月（P＜0.05）.在原代培养肝癌细胞中,来自原发灶的Hep11比来自转移复发灶的Hep12的KLF17表达高,Hep11的KLF17干扰后,迁移和侵袭能力增强（P＜0.05）.结论 KLF17表达可能是肝癌患者预后的独立预测因素.Objective To detect KLF17 expression in hepatocellular carcinoma and to evaluate its effect on the prognosis of Hepatocellular carcinoma （HCC）. Methods Immunohistochemistry was used to detect the expression of KLF17 in hepatocellular carcinoma, and analyze the relationship between KLF17 expression and disease free survival （DFS） and overall survival （OS）of patients. Comparison of the primary cultured Hep11 and Hep12 cells invasion and migration ability, as well as the difference of KLF 17 expression was carried out. SiRNA interfered KLF17 expression of Hep11 ,then transwell chambers were used in the in vitro migration and invison anssay. Results Of 36 patients, 19 patients were score 0 - 2 of KLF17, 17 patients were score 3 - 5. Disease free survival of patients with score 3 - 5 were longer, 39. 3 - 4. 2 months, patients scoree, 0- 2 was 23.4 -4. 9 months （P 〈0. 05 ）. The overall survival of KLF17 score 3 - 5 patients was 46. 9 -3.2 months, patients score 0 -2 of KLF17 was 35.2 -4. 2 months（P 〈0. 05）. Hep12 exhibited a higher invasire and migratory potential than Hep11. There was also a significantly higher expression of KLF17 in Hep11 cells than in Hep11 cells. Down-regulation of KLF17 enhanced migration and invasion of Hep11 cells. Conclusions Expression of KLF17 may be independent predict factors for prognosis of HCC patients.

关 键 词：KLF17 肝癌 预后 

分 类 号：R735.7[医药卫生—肿瘤]