鼻咽癌组织Maspin和GRP78表达与放疗敏感相关性研究  

Expressions of Maspin and GRP78 gene in radiotherapy of nasopharyngeal carcinoma

作　　者：邓香群[1] 叶旭[2] 彭波[3] 阮建阳 

机构地区：[1]邵阳医学高等专科学校组织学与胚胎学教研室,湖南邵阳422000 [2]湖南省肿瘤医院核医学科,湖南长沙410013 [3]衡阳市疾病预防控制中心职业危害因素控制科,湖南衡阳421001 [4]邵阳医学高等专科学校病理教研室,湖南邵阳422000

出　　处：《中华肿瘤防治杂志》2014年第21期1706-1709,共4页Chinese Journal of Cancer Prevention and Treatment

基　　金：湖南省科技厅科技计划(2011FJ4141)

摘　　要：目的研究鼻咽癌组织中Maspin和GRP78的表达,探讨其在放射治疗鼻咽癌的意义。方法选取湖南省肿瘤医院2006-01-13-2006-11-26第一次入院接受放射治疗的60例鼻咽癌患者,运用原位杂交方法检测Maspin mRNA和GRP78mRNA的表达。结果放疗敏感及放疗抗拒鼻咽癌组织中,Maspin mRNA中度和强阳性表达率分别为70.00%和42.50%,χ2=4.038,P=0.044 5,GRP78mRNA中度和强阳性表达率分别为20.00%和47.50%,χ2=4.047,P=0.044 3。在放疗抗拒鼻咽癌组织中,Maspin mRNA中度及强阳性表达率与T分期存在关联性,在T3和T4期中表达率为63.16%,T1和T2期中表达率为23.81%,χ2=6.320,P=0.011 9;在有、无远处转移中的表达率分别为72.73%和31.03%,χ2=4.095,P=0.043 0;GRP78mRNA中度及强阳性表达率与TNM分期也存在关联性,Ⅲ和Ⅳ期GRP78mRNA中度及强阳性表达率为58.62%,Ⅰ和Ⅱ期表达率为18.18%,χ2=5.230,P=0.022 2,与T分期也存在关联性,T3和T4期表达率为68.42%,T1和T2期表达率为28.57%,χ2=6.352,P=0.011 7,在有、无远处转移中的表达率分别为72.73%和37.93%,χ2=3.872,P=0.049 1。结论 Maspin参与放疗抗拒鼻咽癌的发展,GRP78降低鼻咽癌的放疗敏感性。OBJECTIVE To study the expressions of Maspin and GRP78 in the radiotherapy of nasopharyngeal car- einoma, and tb.eir signi.ficances. METHODS Radiotherapies were takett in 60 cases of ttasoplaaryngeal carcinoma from 2006.1.13 to 2006.11.26. In situ hybridization detections were carried out to detect the expression of Maspin and GRP78 mRNA. RESULTS In radiosensitivity and radio-resistance of nasopharyngeal carcinoma, the moderately and strongly positive Maspin mRNA expression rates were 70.00 % and 42.50 % ,x^2= 4. 038,P= 0. 044 5, the moderately and strongly positive GRP78 mRNA expression rates were 20.00% and 47.50%, X^2 =4. 047, P=0. 044 3, there were statistical signif- icances. In the radioresistance of nasopharyngeal carcinoma, Maspin mRNA moderately and strongly positive expression were positively correlated with T stage. In the degree of T3 and T4, it had moderately and strongly positive expression rate of 63.16%. In the degree of T1 and T2 , it had moderately and strongly positive expression rate of 23.81%. X2 ： 6. 320, P： 0. 011 9. In distant metastasis and no distant metastasis the expression rates were 72.73 % and 31.03 %, x^2= 4. 095, P=0. 043 0, the differences had statistical significance; GRP78 mRNA moderately and strongly positive expres- sions were positively correlated with TNM stage. In the degree of Ⅲ and Ⅳ, it had moderately and strongly positive ex- pression rate of 58.62%. In the degree of Ⅰ and Ⅱ , it had moderately and strongly positive expression rate of 18.18%. x^2= 5. 230, P= 0. 022 2. It was also positively correlated with T stage. In the degree of T3 and T4 , it had moderately and strongly positive expression rate of 68.42%. In the degree of T1 and T2, it had moderately and strongly positive expres- sion rate of 28.57%. x^2=6. 352, P=0. 011 7. In distant metastasis and no distant metastasis the expression rates were 72.73 % and 37.93%,x^2= 3. 872, P= 0. 049 1. The differences had statistical significance. CONCLUSION Maspin has participated in the development of radioresist

关 键 词：MASPIN基因 GRP78基因 放射治疗 鼻咽肿瘤 

分 类 号：R739.63[医药卫生—肿瘤]