五倍子水提取物对人牙髓细胞Ⅰ型胶原合成的影响  

The effect of gallnut water extracts on collagenⅠsynthesis in human dental pulp cells

作　　者：卢婧 徐星星[3] 李鑫 贾珍 唐荣银 王菁 

机构地区：[1]第四军医大学口腔医院牙体牙髓病科 军事口腔医学国家重点实验室 [2]解放军第96375部队44卫生队 [3]第四军医大学学员一旅一营二连 [4]第四军医大学口腔医院修复科 军事口腔医学国家重点实验室

出　　处：《牙体牙髓牙周病学杂志》2014年第11期642-644,共3页Chinese Journal of Conservative Dentistry

摘　　要：目的:观察五倍子水提取物对人牙髓细胞Ⅰ型胶原合成的影响。方法:取第6代体外培养的人牙髓细胞分别与终末浓度为5、10μg/m L的五倍子水提取物共同培养3 d后,采用免疫组化染色法观察人牙髓细胞中Ⅰ型胶原的表达情况,并用HPIAS-1000图像分析系统进行定量分析。结果:对照组和不同浓度五倍子水提取物作用的实验组人牙髓细胞中均有Ⅰ型胶原的表达,其免疫反应物质多定位于胞浆。人牙髓细胞经5、10μg/m L浓度的五倍子水提取物作用后,其Ⅰ型胶原的表达水平均明显增高,其中以10μg/m L组的增高程度最大,各组间两两相比差异均有统计学意义(P<0.05)。结论:5、10μg/m L五倍子水提取物均能明显促进人牙髓细胞中Ⅰ型胶原的表达;提示其具有促进牙髓细胞向成牙本质细胞分化的作用。AIM： To observe the effects of gallnut water extracts on collagen I synthesis of human dental pulp cells. METHODS： Cultured human dental pulp cells of passage 6 were treated with 5 μg / m L and 10 μg / m L of gallnut water extracts for 3 d respectively. Collagen I expression of the cells was detected by immunocytochemistry method. The quantitative data was obtained by HPIAS- 1000 image analysis system. RESULTS： Collagen I protein was expressed in of both gallnut water extract treated dental pulp cells and control cells,and located mainly in cytoplasm. Gallnut water extracts treatment dose- dependently increased collagen I protein expression（ P 〈0. 05）. CONCLUSION： Both 5 μg / m L and 10 μg / m L gallnut water extracts can enhance collagen I protein expression in human dental pulp cells,indicating that it is potential to promote dental pulp cells to differentiate into odontoblasts.

关 键 词：五倍子水提取物 体外培养 人牙髓细胞 Ⅰ型胶原 

分 类 号：R780.2[医药卫生—口腔医学]