表面蛋白抗原P及其在变异链球菌生物膜形成中的作用  

Function of surface protein antigen P in the biofilm formation of Streptococcus mutans

作　　者：张剑英[1,2] 凌均棨[1,2] 

机构地区：[1]中山大学光华口腔医学院.附属口腔医院牙体牙髓病科 [2]广东省口腔医学重点实验室,广州510055

出　　处：《国际口腔医学杂志》2015年第1期111-113,共3页International Journal of Stomatology

基　　金：国家自然科学基金(81371132)

摘　　要：变异链球菌表面蛋白抗原(SPA)P是一类介导细菌黏附和生物膜形成的重要的黏附毒力因子,具有高度保守性。SPAP含有1 561个氨基酸残基,其线性结构氨基酸序列由前导肽区、N端、A区、V区、P区、C端和细胞壁锚着端组成。SPAP具有的淀粉样纤维特性,在细菌生物膜形成中十分重要。SPAP可与牙面获得性膜中的唾液成分结合,介导变异链球菌对牙面的初始黏附。SPAP通过分选酶转移肽共价结合到细菌胞壁表面,在内源性的表面蛋白释放酶作用下又可从细菌胞壁表面释放出来,从而使变异链球菌生物膜降解。本文就SPAP的结构、淀粉样纤维特性,变异链球菌黏附,SPAP各区在生物膜形成中的作用等研究进展作一综述,明确其生物学特性,对于龋病病因学和龋病防治的意义不言而喻。Surface protein antigen（SPA） P is an important virulence factor with high conservation that serves a vital function in mediating the adhesion and biofilm formation of Streptococcus mutans（S.rnutans）. The SPAP linear sequence comprises a leader peptide, N-terminal, A, V, P, and C-terminal regions, and a cell wall-anchoring segment counting up to 1 56i amino acid residues. The SPAP has an amyloid fibrous property and is thus important in the biofilm formation process. SPAP is closely associated with the development of dental caries because of its interaction with host salivary components, matrix proteins, and other microorganisms. The SPAP covalently combines with the cell wall of S.mutans under the influence of sortase. S.mutans also produces a surface protein-releasing enzyme that degrades SPAP and mediates cell release from biofilms. This review describes the structure and the amyloid fibrous property of SPAP with emphases on studies aiming to characterize the effects of SPAP on S.mutans biofilm formation. This review also aims to identify biological characteristic cues in etiology and potential therapeutic applications of dental caries.

关 键 词：表面蛋白抗原P 变异链球菌 生物膜形成 

分 类 号：R780.2[医药卫生—口腔医学]