胃癌组织中钙网织蛋白的表达及其与临床病理特征的关系  

Expression of calreticulin in gastric carcinoma and its correlation with clinicopathology

作　　者：黄红丽[1] 聂玉强[1] 李瑜元[1] 杜艳蕾[1] 

机构地区：[1]广州医科大学附属广州市第一人民医院消化内科广州市消化病重点实验室,510180

出　　处：《中华生物医学工程杂志》2014年第3期195-199,共5页Chinese Journal of Biomedical Engineering

基　　金：广东省自然科学基金面上项目（10151006001000016）;广州市医药卫生科技项目（20131A011028）

摘　　要：目的 观察钙网织蛋白（CRT）在胃癌组织中的表达水平及其与临床病理的关系.方法 收集2009年4月至2010年8月本院外科手术切除并经病理确诊的44例新鲜胃癌组织及癌旁正常胃组织,采用实时荧光定量PCR和免疫组织化学方法分别检测44例胃癌及癌旁正常胃组织中CRT的mRNA和蛋白表达水平,分析其表达情况与临床病理学特征之间的关系.结果 胃癌组织中CRT的mRNA表达水平低于正常胃组织[36.50（21.72,71.47）比48.19（20.84,139.20）,P=0.031].CRT蛋白主要定位于细胞质中,在胃癌组织中其阳性表达率明显低于正常胃组织[34.09％（15/44）比77.27％ （34/44）,P=0.000].胃癌组织中CRT mRNA和蛋白的低表达均与胃癌的分化程度、淋巴结转移等临床病理因素密切相关（均P＜0.05）,与患者的年龄、性别、肿瘤大小、TNM分期无关（均P＞0.05）.结论 胃癌组织中CRT低表达,且与淋巴结转移、胃癌分化程度密切相关,是一项有潜在价值的肿瘤相关标志物.Objective To investigate protein expression of calreticulin （CRT） in gastric carcinoma tissues and examine its correlation with clinicopathology.Methods Fresh gastric carcinoma tissues and corresponding paraneoplastic normal tissues of 44 patients admitted to our hospital from April 2009 to August 2010 were collected.The mRNA and protein levels of CRT expression in 44 cases of gastric carcinoma tissues and corresponding paraneoplastic normal tissues were determined by quantitative real-time PCR and immunohistochemist~.Meanwhile,the correlations between CRT expression and clinicopathological factors were observed.Results The mRNA level of CRT expression in gastric carcinoma tissues was lower compared with paraneoplastic normal gastric tissues [36.50 （21.72,71.47） vs 48.19 （20.84,139.20）,P=0.031].The protein expression of CRT was found mainly locating in cytoplasm,and the positive rate of CRT protein in gastric carcinoma tissues was significantly lower than that in normal gastric mucosa [34.09％ （15/44）vs 77.27％ （34/44）,P=0.000].Moreover,low expression of CRT mRNA and protein in gastric carcinoma tissues was correlated with tumor differentiation degree and lymph node metastasis （all P＜0.05）,but was not correlated with age,sex,tumor size or TNM stages （all P＞0.05）.Conclusions Low expression of CRT is found in gastric carcinoma tissues and correlated well with lymph node metastasis and tumor differentiation degree.CRT may be a potential tumor marker for gastric carcinoma.

关 键 词：胃肿瘤 钙网织蛋白 病理学 临床 

分 类 号：R735.2[医药卫生—肿瘤]