富氢水和乳果糖对环磷酰胺处理小鼠的抗突变作用  

Anti-mutagenic effect of hydrogen-rich water and lactulose on mice treated with cyclophosphamide

作　　者：欧阳永长[1] 温伟文[1] 刘玉[1] 

机构地区：[1]广州医科大学基础学院生化教研室,510182

出　　处：《中华生物医学工程杂志》2014年第4期294-297,共4页Chinese Journal of Biomedical Engineering

基　　金：广州市属高校科研项目（10A161）

摘　　要：目的 探讨饮用富氢水和产氢营养因子乳果糖的抗突变作用.方法 36只昆明小鼠按随机数字表分为6组,每组6只.空白对照组（A组）、富氢水处理组（B组）和乳果糖处理组（C组）：这3组小鼠正常喂养3d后,第4～6天分别使用蒸馏水、富氢水、50％乳果糖灌胃（20 ml/kg、1次/d）;抗生素对照组（D组）、抗生素＋富氢水处理组（E组）和抗生素＋乳果糖处理组（F组）：这3组小鼠第1～3天随意饮用含抗生素的水,第4～6天分别使用蒸馏水、富氢水、50％乳果糖灌胃（20 ml/kg、1次/d）.末次灌胃后小鼠腹腔注射环磷酰胺（0.04 g/kg）,24 h后处死小鼠,进行骨髓细胞学检查,计算各组小鼠骨髓嗜多染红细胞微核率.结果 A、B、C、D、E、F组小鼠骨髓嗜多染红细胞微核率分别为（16.00±0.67）‰、（15.11±0.25）‰、（10.17±0.35）‰、（22.39±0.51）‰、（15.44±0.44）‰、（18.67±0.37）‰.与A组比较,C组微核率显著减少,D组微核率显著增加（均P＜0.01）.与D组比较,E组和F组微核率显著减少（均P＜o.o1）.与C组比较,F组微核率显著增加（P＜0.01）.结论 富氢水和乳果糖均可拮抗环磷酰胺的致突变作用,抗生素预处理可以显著降低乳果糖的抗突变作用.Objective To evaluate the anti-mutagenic effect of potable hydrogen-rich water and lactulose,a hydrogen-producing prebiotics.Methods Thirty-six Kungming mice were randomly divided into 6 groups （n=6 each） by random number table.Blank control group （group A）,hydrogen-rich water treatment group （group B）,and lactulose treatment group （group C）,after 3 days of normal feeding,these 3 groups received gavage of distilled water,hydrogen-rich water and 50％ lactulose （20 ml/kg,once-daily）,respectively from days 4 to 6.Another 3 groups were antibiotic control group （group D）,antibiotic ＋ hydrogen-rich water treatment group （group E） and antibiotic ＋ lactulose treatment group （group F）,mice of these 3 groups had free access to antibiotic-containing water from days 1 to 3,then received garage of distilled water,hydrogen-rich water and 50％ lactulose （20 ml/kg,once-daily）,respectively from days 4 to 6.After the last gavage,the mice were intraperitoneally injected with cyclophosphamide （0.04 g/kg）,and executed at 24 h later.The bone marrow cells were detected and micronuclei rate of polychromatic erythrocytes of mice bone marrow in each group was calculated.Results The micronuclei rate of polychromatic erythrocytes in groups A,B,C,D,E and F was （16.00±0.67）‰,（15.11±0.25）‰,（10.17±0.35）‰,（2239±0.51）‰,（15.44±0.44）‰,and （18.67±0.37）‰ respectively.Compared with group A,the micronuelei rate was significantly lower in group C,but significantly higher in group D （both P＜0.01）.Compared with group D,the micronuclei rates in groups E and F were significantly lower （both P＜0.01）.Compared with Group C,the micronuclei rate in group F was significantly elevated （P＜0.01）.Conclusion Both hydrogen-rich water and lactulose have antimutagenic effect against cyclophosphamide,and antibiotic pretreatment can significantly reduce the antimutagenic effect of lactulose.

关 键 词：乳果糖 微核试验 环磷酰胺 富氢水 突变 

分 类 号：TQ460.1[化学工程—制药化工]