荧光定量RCR检测比格犬雌激素β受体mRNA在间情期与发情期表达量的差异  

The expression difference between estrus and non-estrus of ERβ mRNA of beagles by real-time quantitative PCR

作　　者：钟睿[1,2] 甘艺[2,3] 任秀梅[2] 许琴[2] 赵彦斌[2] 刘冰[2] 孙兆增[2] 朱宇旌[1] 栾新红[1] 胡仲明[2] 张勇[1] 曾林[2] 

机构地区：[1]沈阳农业大学畜牧兽医学院,沈阳110866 [2]军事医学科学院实验动物中心,北京100071 [3]黑龙江八一农垦大学动物科技学院,黑龙江大庆163319

出　　处：《中国比较医学杂志》2014年第11期15-19,共5页Chinese Journal of Comparative Medicine

基　　金：国家自然科学基金资助项目(31372271);国家自然科学基金资助项目(31172164)

摘　　要：目的对比格犬发情期与间情期ERβ基因在性腺组织器官的变化状况进行荧光定量,明确ERβ基因在发情期与间情期下丘脑-垂体-性腺轴上的表达量差异情况,为深入研究比格犬发情机制提供基础。方法作为调控生殖的关键基因,ERβ基因位于比格犬下丘脑-垂体-性腺轴上,因此分别取处于发情期和间情期的比格犬,提取下丘脑、垂体、卵巢和子宫的RNA,反转录后对ERβ基因mRNA的表达量进行荧光定量PCR检测。结果间情期比格犬卵巢、子宫、垂体、下丘脑内ERβ基因mRNA的表达量分别是发情期比格犬卵巢、子宫、垂体、下丘脑内ERβ基因mRNA表达量的0.35倍、0.17倍、0.44倍、0.43倍。结论 ERβ基因在处于发情期的比格犬下丘脑-垂体-性腺轴内表达量均上调。Objective To quantitative the changing information of estrogen receptor β gene which was in tissue and organ of sex gland during oestrus and dioestrus of Beagles, and to show the different expression situation of hypothalamus-pituitary-gonad axis during oestrus and dioestrus, and providing the basic of theory to research deeply the mechanism of heat of Beagles. Methods As the key gene in regulation reproduction, ERβ gene is located in hypothalamus-pituitary-gonad axis, so using Beagles which was in oestrus and dioestrus, and extract the RNA from hypothalamus.pituitary.ovary and uterus respectively,after reverse transcription we detected the expression of ERβ gene byreal-time quantitative PCR. Results The expression of ERβ gene mRNA from ovary, uterus, pituitary, hypothalamus of Beagles which was in dioestrus was 0. 35 times, 0. 17 times, 0.44 times and 0. 43 times than the expression of ERβ gene mRNA from ovary, uterus, pituitary, hypothalamus of Beagles which was in oestrus. Conclusion The expression of ERβ gene was up-regulation in hypothalamus-pituitary-ovary axis of Beagles which was in oestrus.

关 键 词：ERβ基因 发情期 间情期 比格犬 

分 类 号：R332[医药卫生—人体生理学]