肾上腺素受体在乳腺癌细胞中的表达及其对细胞增殖的影响  

Expression of adrenergic receptors in breast cancer cells and their influence on cell proliferation

作　　者：高德宗[1] 刘兴莉[2] 马忠兵[1] 

机构地区：[1]山东大学第二医院乳腺外科,山东济南250033 [2]山东大学齐鲁儿童医院,检验科,山东济南250022

出　　处：《中国普通外科杂志》2014年第11期1501-1505,共5页China Journal of General Surgery

基　　金：山东大学自主创新基金资助项目(2012TS185)

摘　　要：目的:探讨肾上腺素受体在乳腺癌细胞中的表达以及将其阻断后对乳腺癌细胞增殖的变化。方法:用免疫细胞化学方法分别检测乳腺癌MCF-7(ER阳性)和MDA-MB-231(ER阴性)细胞中α1、α2、β1、β2肾上腺素受体的表达;MTT法分别检测去甲肾上腺素、卡维地洛(非特异性肾上腺素受体阻断剂)或两种联合作用后两种乳腺癌细胞的增殖情况。结果:MCF-7和MDA-MB-231细胞均有α与β肾上腺素受体表达,但受体亚型表达水平不同,MCF-7细胞α1、β1受体表达相对较强,α2、β2受体表达相对较弱,MDA-MB-231细胞则与MCF-7相反;卡维地洛能明显抑制两株乳腺癌细胞增殖,并呈一定的时间与浓度依赖趋势,部分差异有统计学意义(均P<0.05);去甲肾上腺素能明显促进两种乳腺癌细胞的增殖,但与卡维地洛共同作用后,其促细胞增殖作用被明显拮抗,并呈浓度依赖性,差异均有统计学意义(均P<0.05)。结论:乳腺癌细胞同时不同程度地表达α、β肾上腺素受体亚型,非特异性阻断肾上腺素受体可有效抑制乳腺癌细胞的增殖。Objective：To investigate the expression of adrenergic receptors in breast cancer cells and changes in breast cancer cell proliferation after their blockage.Methods：The expressions of adrenergic α1-,α2-,β1 and β2-receptor in breast cancer MCF-7（ER-positive） and MDA-MB-231（ER-negative） cells were examined by immunocytochemical staining.The proliferation of the two types of breast cancer cells was analyzed by MTT assay after they were exposed to norepinephrine and carvedilol（non-selective blocker of adrenergic receptors） alone,or in combination,respectively.Results：The MCF-7 and MDA-MB-231 cells both expressed the adrenergic a- and β-receptor,but the expression levels of the adrenergic-receptor subgroups were different between them.The α1- and β1-receptor showed a relatively strong expression while α2- and β2-receptor showed a relatively weak expression in MCF-7 cells,but there was an opposite pattern of expression in MDA-MB-231 cells.The proliferation of both types of breast cancer cells was significantly inhibited by carvedilol treatment,with a certain time- and concentration dependent trend toward higher inhibition,and some of the differences had statistical significance（all P〈0.05）;the proliferation of both types of breast cancer cells were significantly increased after norepinephrine treatment,which was concentration-dependently antagonized by carvedilol co-treatment,and all the differences reached statistical significance（all P〈0.05）.Conclusion：Both adrenergic a- and β-receptor are present in breast cancer cells with varying levels of expression,and non-selective blockage of adrenergic receptors can effectively suppress the proliferation of breast cancer cells.

关 键 词：乳腺肿瘤 受体 肾上腺素能 细胞增殖 

分 类 号：R737.9[医药卫生—肿瘤]