青黛对结肠黏膜成纤维细胞的影响及作用机制  

Mechanism and influence of Indigo Naturalis on the functions of colonic fibroblasts

作　　者：陈小璇[1] 代剑平[1] 朱丹霞[1] 王革非[1] 李康生[1] 

机构地区：[1]汕头大学医学院微生物与免疫学教研室,广东汕头515041

出　　处：《胃肠病学和肝病学杂志》2014年第11期1290-1295,共6页Chinese Journal of Gastroenterology and Hepatology

基　　金：国家自然科学基金资助项目(81374040;31170852;81001322;81172795)

摘　　要：目的探讨青黛对结肠黏膜成纤维细胞的影响及其作用机制。方法提取青黛,并命名为水提物、醇提物和总提物,试验方法有羟脯氨酸测定、明胶酶谱分析、qRT-PCR、ELISA和Western blotting等。结果水提物可促进小鼠结肠黏膜成纤维细胞增殖和胶原积累,提高MMPs活性和TIMP-1/-2生成,上调TGFβ1和CTGF水平,并激活PI3K/Akt、PKC及ERK/JNK/p38 MAPK信号通路。醇提物能抑制成纤维细胞增殖,减少TGFβ1、CTGF、IL-6、IL-1和TNF-α转录和释放,降低p-Akt和p-p38水平。总提物能促进成纤维细胞增殖和胶原积累,提高MMPs活性和TIMP-1/-2生成,上调TGFβ1和CTGF水平,激活PKC、ERK MAPK通路。结论青黛水提物和醇提物对成纤维细胞增殖和胶原积累的作用相反,其总提物促进溃疡治愈可能依赖于PKC及ERK MAPK信号通路。Objective To investigate the influence of Indigo Naturalis on the function of colonic fibroblasts relevant to ulcer healing.Methods Indigo Naturalis was extracted,and named aqueous etract,alcoh-oletract and the total rextract,hydroxyproline assay,gelatin zymography,qRT-PCR,ELISA and Western blotting were used.Results The aqueous extract could promote mice colonic fibroblast proliferation and collagen accumulation,increased the production of MMPs,TIMP-1/-2,TGFβ1 and CTGF,and activated PI3K/Akt,PKC,ERK/JNK/p38 MAPK pathways.The alcoholic extract could inhibit fibroblast proliferation,decreased the production of TGFβ1,CTGF,IL-6,IL-1,TNF-α,and the levels of p-Akt and p-p38.The total extract could promote fibroblast proliferation and collage accumulation,increased the production of MMPs,TIMP-1/-2,TGFβ1 and CTGF,and activated PKC and ERK MAPK pathway.Conclusion Indigo Naturalis contains two different kinds of ingredients with opposite effects for fibroblast proliferation and collage accumulation; the total extract promotes fibroblast proliferation and collage accumulation via activating PKC and ERK MAPK signal pathway.

关 键 词：青黛 成纤维细胞 溃疡愈合 

分 类 号：R574.62[医药卫生—消化系统]