阿托伐他汀对人胆管癌QBC939细胞系侵袭的影响  

Effects of Atorvastatin on invasion abilities in human cholangiocarcinoma QBC939 cells

作　　者：郑龙志[1] 林伟[1] 陈有挺[2] 石铮[2] 何庆良[2] 

机构地区：[1]莆田学院附属医院普通外科,福建莆田351100 [2]福建医科大学附属第一医院普通外科

出　　处：《胃肠病学和肝病学杂志》2014年第11期1344-1346,共3页Chinese Journal of Gastroenterology and Hepatology

摘　　要：目的探讨阿托伐他汀(ATV)对人胆管癌QBC939细胞系侵袭的影响及其可能作用机制。方法应用细胞培养技术培养人胆管癌QBC939细胞,经不同浓度的ATV处理后,以Matrigel侵袭实验和半定量RT-PCR检测ATV对QBC939细胞内Rho C mRNA表达的影响情况。结果 Matrigel侵袭实验显示,经10μmol/L、25μmol/L、50μmol/L干预48 h后的QBC939细胞,随着浓度的增加,其体外侵袭能力明显减弱(P<0.01);半定量RT-PCR测定显示,ATV作用48 h后,QBC939细胞中Rho C的表达改变并不明显。结论 ATV可减弱人胆管癌QBC939细胞体外侵袭能力。Objective To investigate the effects and possible mechanisms of Atorvastatin （ATV） on invasion abilities in human cholangiocarcinoma QBC939 cells.Methods The human cholangiocarcinoma QBC939 cells was incubated with different concentrations of atorvastatin for 48 hours.The influcance on mRNA expression of RhoC was detected by invasion assays and semiquantitative RT-PCR.Results Matrigel invasion assay showed that the vitro invasire ability of QBC939 reduced sighificanty with increasing concentration of ATV after 48 hours.Semiquantitative RT-PCR analysis showed that the expression of RhoC was not significantly altered among the groups.Conclusion ATV can inhibits invasion of human cholangiocarcinoma QBC939 cells.

关 键 词：阿托伐他汀 QBC939细胞 肿瘤侵袭 

分 类 号：R735.8[医药卫生—肿瘤]