Pokemon对乳腺癌MCF-7细胞侵袭迁移能力的影响  

The Effect of Pokemon on the Invasion and Migration Ability of MCF-7 Cells

作　　者：祖旭宇[1] 谭莉[1] 周茜[1] 王艺蓁 

机构地区：[1]南华大学附属第一医院临床医学研究所,湖南衡阳421001

出　　处：《中南医学科学杂志》2014年第6期545-548,共4页Medical Science Journal of Central South China

基　　金：国家自然科学基金项目(81272355);湖南省教育厅青年项目资助(12B108)

摘　　要：目的建立稳定表达pokemon乳腺癌细胞系,并观察pokemon对乳腺癌细胞系MCF-7侵袭转移能力的影响。方法构建表达pokemon慢病毒载体,将Pokemon表达慢病毒载体转染MCF-7细胞,通过Western blot方法检测稳定性表达pokemon MCF-7细胞中Pokemon-GFP融合蛋白的表达情况,应用Transwell试验及划痕实验观察pokemon的高表达对MCF-7细胞侵袭迁移能力的影响。结果成功建立稳定表达pokemon的MCF-7细胞系,pokemon可抑制MCF-7细胞侵袭能力。结论 Pokemon可抑制MCF-7细胞侵袭能力。Objective To establish MCF-7 cells with stablely ectopic pokemon expression and to assess the effect of pokemon on the invasion and migration of MCF-7 cells. Methods Immunofluorescence and Western blot were used to screen the MCF-7 cell clones with ectopic pokemon expression. Transwell and wound healing analysis were utilized to deter- mine the effect of pokemon on the invasion and migration of MCF-7 cells. Results The MCF-7 cell line with stablely ec- topic pokemon expression was established and Pokemon was demonstrated to suppress the invasion ability of MCF-7 cells. Conclusion Pokemon could suppress the invasion ability of MCF-7 cells.

关 键 词：POKEMON 慢病毒载体 MCF-7细胞 细胞侵袭能力 

分 类 号：R737.9[医药卫生—肿瘤]