高纬度地区大豆蛋白含量及氨基酸组分表型鉴定与聚类分析  

Phenotype Identification and Cluster Analysis of Soybean in Upper Latitudes Regions Based on Protein Content and Amino Acid Composition

作　　者：邱红梅[1] 杨春明[1] 高淑芹[1] 侯云龙[1] 马晓萍[1] 孙星邈[1] 郑宇宏[1] 王跃强[1] 王曙明[1] 

机构地区：[1]吉林省农业科学院大豆研究所/大豆国家工程研究中心,长春130033

出　　处：《植物遗传资源学报》2014年第6期1202-1208,共7页Journal of Plant Genetic Resources

基　　金："863"计划课题(2012AA101106);国家自然科学基金(31401404);现代农业产业技术体系专项(CARS-04-PS11);吉林省自然基金(20140101142JC)

摘　　要：对国内外高纬度地区的240份大豆种质资源的蛋白及16个氨基酸组分进行测定,通过遗传多样性、因子和聚类分析,进行了表型鉴定及基因型分类。结果表明,供试大豆种质蛋白及氨基酸组分变异较丰富,遗传多样性程度较高。根据因子分析,将筛选到的3个公因子进行聚类分析,可将供试种质资源分为7类。蛋白含量从高到低的顺序为类群Ⅶ>类群Ⅵ>类群Ⅴ>类群Ⅱ>类群Ⅰ>类群Ⅲ>类群Ⅳ。12个氨基酸组分的变化趋势与蛋白一致。类群Ⅶ和类群Ⅵ为高蛋白遗传群体,可作为高蛋白基因聚合育种的亲本材料。通过前期分析,筛选到24份高蛋氨酸资源,包括有公野04L-141、龙品03-311、Proto、和龙油太、猫眼豆、茶色豆、紫花2号、东农48等,可为高蛋氨酸种质创新提供材料基础。To identify the phenotypes and to classify the genotypes through genetic diversity,factors and cluster analysis,the content of protein and 16 amino acids was measured in 240 soybean core collections in upper latitude regions.The results showed that there were higher genetic variation and diversity for the content of protein and 16 amino acids in 240 soybean core collections.Three common factors were screened for clustering analysis according to the factor analysis.Seven genetic groups were divided through clustering analysis for 240 soybean core collections.The sequence for protein content from high to low acted as group 7〉 group 6〉 group 5〉 groups 2〉 groups 1〉 groups 3 〉group 4.Change trend of 12 amino acids composition was in accordance with protein content.Group 7and 6 for high protein genetic groups would be used as parent materials for gene polymerization breeding.24 high methionine germplasm resources were identified by analysis,including Gongye 04L-141,Longpin 03-311,Proto,Helongyoutai,Maoyandou,Chasedou,Zihua 2,Dongnong 48.These germplasm resources were the material basis for innovation of high methionine germplasm.

关 键 词：大豆 蛋白 氨基酸组分 遗传多样性 聚类分析 

分 类 号：S565.1[农业科学—作物学]