白细胞介素4调节人脐带间充质干细胞的增殖和造血支持能力  

Interleukin-4 regulates phonotype,proliferation and hematopoietic supporting capacity of human umbilical cord mesenchymal stem cells

作　　者：杨舟鑫[1] 韩之波[2] 贾兵兵[1] 王国付[1] 

机构地区：[1]浙江医院浙江省老年医学重点实验室,浙江杭州310013 [2]中国医学科学院北京协和医学院血液学研究所血液病医院实验血液学国家重点实验室,天津300020

出　　处：《中国病理生理杂志》2014年第11期2048-2053,共6页Chinese Journal of Pathophysiology

基　　金：国家自然科学基金资助项目(No.31301139;No.31201040)

摘　　要：目的:间充质干细胞(MSC)被认为在多种自身免疫系统疾病和移植物抗宿主病中有良好的应用前景,但是其在各种炎症因子作用下生物学功能的变化仍然值得进一步研究。本文探讨了白细胞介素4(IL-4)对脐带间充质干细胞(UC-MSC)的多种生物学特性以及造血支持作用的影响。方法:IL-4刺激UC-MSC后,使用流式细胞术检测UC-MSC免疫表型;使用MTT检测细胞活力改变,并使用Brd U-ELISA的方法,检测其增殖能力;使用油红O和茜素红染色来检测UC-MSC成脂和成骨分化;使用实时荧光定量PCR的方法,检测UC-MSC中mRMA表达的差异;收集IL-4刺激的UC-MSC的上清,检测其在促进CD34+细胞集落形成能力的变化。结果:IL-4刺激后,UC-MSC表面的CD11b、CD19、CD34、CD45、CD73、CD90、CD105、人类白细胞抗原(HLA)-DR和HLA-ABC并未有明显改变。MTT和Brd U-ELISA都表明,IL-4处理组UC-MSC的增殖在72 h后相对于未处理组明显下降(P<0.05)。IL-4处理后,UC-MSC成脂和成骨分化能力未检测到明显的变化。此外,IL-4可以明显增强UC-MSC中IL-6mRNA表达(P<0.01),增强UC-MSC培养上清的促CD34+细胞粒-巨噬细胞集落形成单位(CFU-GM)的集落形成能力(P<0.01)。结论:IL-4可以抑制UC-MSC增殖,促进UC-MSC造血支持能力。AIM： To explore the effects of interleukin-4（ IL-4） on the biological characteristics and hematopoietic supporting effects of umbilical cord mesenchymal stem cells（ UC-MSC）. METHODS： The phenotype of UC-MSC was detected by flow cytometry after IL-4 stimulation,and the proliferation ability of UC-MSC was measured by Brd UELISA. Oil red O and alizarin red were used to observe the ability of differentiation. The mRNA expression in UC-MSC was determined by real-time fluorescence quantitative PCR. The culture medium isolated from UC-MSC was used to analyze the ability in promoting colony formation. RESULTS： After IL-4 stimulation,the expression of CD11 b,CD19,CD34,CD45,CD73,CD90,CD105,HLA-DR and HLA-ABC was unchanged. IL-4 inhibited the proliferation of UC-MSC,but no difference was detected on osteogenic and adipogenic differentiation. The culture medium from IL-4-induced UC-MSC possessed strong ability for promoting CD34＋colony formation ability. CONCLUSION： IL-4 inhibits the proliferation of UC-MSC and enhances its hematopoietic supporting ability.

关 键 词：间充质干细胞 白细胞介素4 

分 类 号：R363[医药卫生—病理学]