Sp3对β-catenin基因转录调控作用初探  

Effect of Sp3 on regulation of β-catenin transcription

作　　者：黄兰姗 黄子凌 冯振博[1] 陈罡[1] 危丹明[1] 

机构地区：[1]广西医科大学第一附属医院病理科,广西南宁530021

出　　处：《中国病理生理杂志》2014年第11期2066-2070,共5页Chinese Journal of Pathophysiology

基　　金：广西科技攻关基金资助项目(No.1298003-2-5);广西"新世纪十百千人才工程"专项基金资助项目(No.2007214)

摘　　要：目的:观察转录因子Sp3对β-catenin启动子转录活性的影响,探讨Sp3与Wnt/β-catenin信号通路的关系。方法:采用脂质体法将Sp1和(或)Sp3转录因子表达质粒单独/共同与β-catenin启动子(-410/-1bp)报告基因质粒瞬时转染HEK293T细胞;通过双萤光素酶报告基因实验检测报告基因转录活性的变化。结果:(1)Sp1表达质粒在0.4μg剂量时能提高启动子的活性2.4倍,Sp3表达质粒在0.4μg剂量时能显著提高启动子的活性5.3倍。(2)0.4μg总量的Sp1与Sp3表达质粒共转染293T细胞,随着Sp3/Sp1比例的递增,β-catenin启动子转录活性无明显变化。(3)共同转染Sp1 0.3μg与Sp3 0.1μg时,启动子活性提高3.5倍,比单独转染的转录活性强。结论:Sp3能促进β-catenin基因启动子(-410/-1 bp)的转录,Sp1的转录激活作用则较弱;在转录过程中Sp1和Sp3可能存在协同作用;Sp3可能在转录水平调控β-catenin的表达从而影响Wnt/β-catenin信号通路。AIM： To investigate the effect of Sp3 on the transcriptional regulation of the β-catenin promoter and reveal the association of Sp3 and Wnt / β-catenin. METHODS： Sp1 and / or Sp3 expression plasmids and β-catenin reporter plasmid（- 410 /- 1 bp） were transiently transfected into HEK293 T cells with liposomes. Dual luciferase reporter gene assay was employed to detect the alteration of transcription activity of β-catenin promoter. RESULTS： Sp1 expression plasmid（ 0. 4 μg） induced 2. 4-fold increase in the promoter activity,while Sp3 expression plasmid（ 0. 4 μg） significantly induced 5. 3-fold increase. The promoter activity remained unchanged with the increase in Sp3 / Sp1 after co-transfection of total 0. 4 μg Sp1 and Sp3 expression plasmids. Co-transfection of Sp1 expression plasmid（ 0. 3 μg） and Sp3 expression plasmid（ 0. 1 μg） induced 3. 5-fold increase in the promoter activity,stronger than the individual transfection. CONCLUSION： Sp3 may activate the transcription of β-catenin gene（- 410 /- 1 bp）,while Sp1 shows weaker activated function.Co-transfection of Sp1 and Sp3 gains little effect on the transcription,while the synergistic effect between Sp1 and Sp3 may exist. Sp3 may influence Wnt /β-catenin pathway via interaction with β-catenin during transcription period.

关 键 词：转录因子Sp3 转录因子SP1 Β-CATENIN 转录调控 

分 类 号：R363[医药卫生—病理学]