胰岛素样生长因子-1对甲状旁腺激素介导成骨细胞增殖及成骨活性的影响  

Effect of insulin-like growth factor-1 on the osteoblast proliferation and activity mediated by parathyroid hormone

作　　者：刘冰[1] 卢蕾阳 高飞[1] 杨静[1] 

机构地区：[1]山西医科大学第一医院内分泌科,太原030001

出　　处：《中国骨质疏松杂志》2014年第11期1298-1301,共4页Chinese Journal of Osteoporosis

基　　金：山西省科技厅科技发展计划项目(20130313022-4)

摘　　要：目的通过构建乳鼠颅盖骨成骨细胞分离培养与鉴定方法,研究胰岛素样生长因子-1(Insulin-like growth factor-1,IGF-1)及IGF-1联合重组人甲状旁腺激素1-34(recombinant human parathyroid hormone 1-34,rhPTH1-34)对成骨细胞增殖及I型胶原蛋白mRNA表达的影响。方法培养乳鼠成骨细胞,并观察其形态及功能,以原代培养乳鼠成骨细胞为实验模型,分空白组、PTH组、IGF-1组及不同浓度IGF-1作用的PTH介导的成骨细胞组(0、10、50、100 ng/L)。通过不同剂量的胰岛素样生长因子-1(0、10、50、100 ng/L)联合10-9mol/L重组人甲状旁腺素刺激体外培养的成骨细胞,用噻唑蓝(MTT)法检测细胞的增殖能力,RT-PCR法检测成骨细胞I型胶原蛋白mRNA的表达。结果 PTH和IGF-1均可促进成骨细胞增殖;IGF-1联合PTH可以促进成骨细胞增殖,且具有剂量依赖性。PTH联合IGF-1使成骨细胞增殖能力增强、I型胶原蛋白mRNA表达增强。IGF-1可以促进成骨细胞I型胶原蛋白mRNA的表达,且具有剂量依赖性。IGF-1可以促进成骨细胞I型胶原蛋白mRNA的表达,且具有剂量依赖性。结论 PTH与IGF-1均可刺激成骨细胞增殖和分化,IGF-1可促进PTH对成骨细胞的分化、增殖。两者合用其作用增强,有协同促进作用。Objective Using the rat calvaria bone osteoblast isolation and identification method, to study the effect of insulin-like growth factor-1 （IGF-1） and IGF-1 combined with recombinant human parathyroid hormone 1-34 （rhPTH1-34） on the osteoblast proliferation and the mRNA expression of type-1 collagen.Methods Osteoblasts from new born rats were cultured and their morphology and function were observed.Rat osteoblasts in primary culture were divided to blank control group, PTH group, IGF-1 group, and different concentrations of IGF-1 combined with PTH group （0, 10, 50, and 100 ng/L）.The osteoblasts were stimulated using different concentrations of IGF-1 （ 0, 10, 50, and 100 ng/L ） combined with 10 -9 mol/L of rhPTH1-34. Proliferation of the cells was assessed using MTT colorimetric assay and the mRNA expression of type-1 collagen was examined using RT-PCR.Results PTH and IGF-1 can both promote proliferation of osteoblasts.IGF-1 combining with PTH can promote osteoblast proliferation in a dose dependent manner.They together can increase the expression of type-1 collagen mRNA of the osteoblast.IGF-1 can promote osteoblast type 1 collagen mRNA expression in a dose dependent manner.Conclusion Both PTH and IGF-1 can stimulate the proliferation and differentiation of osteoblasts. IGF-1 can promote PTH-mediated osteoblast differentiation and proliferation.Using the two of them can improve the proliferation effect and they may have synergistic effect.

关 键 词：重组人甲状旁腺激素1-34 胰岛素样生长因子-1 成骨细胞 增殖 I型胶原蛋白mRNA 

分 类 号：R362[医药卫生—病理学]