BTG1在非小细胞肺癌中的表达及其对肺癌细胞增殖、凋亡和侵袭转移的作用  

The effect of BTG1 expression on proliferation,apoptosis and invasion of non-small cell lung cancer cells

作　　者：孙国贵[1] 张洁[2] 崔大为[3] 李成林[4] 杨从容[4] 张钧[4] 程云杰[4] 

机构地区：[1]唐山市人民医院放化疗科,河北唐山063000 [2]唐山市人民医院病理科,063000 [3]唐山市工人医院泌尿外科,063000 [4]河北医科大学第四医院放疗科,050017

出　　处：《临床肿瘤学杂志》2014年第11期972-976,共5页Chinese Clinical Oncology

摘　　要：目的探讨B细胞易位基因1(BTG1)在非小细胞肺癌(NSCLC)组织中的表达及其对NSCLC细胞生物学行为的影响。方法采用免疫组织化学染色和Western blotting分别检测82例NSCLC及38例癌旁正常组织中BTG1蛋白的表达。采用慢病毒转染建立BTG1过表达的NSCLC H1299细胞株。采用实时荧光定量PCR和Western blotting检测BTG1转染后H1299细胞株中BTG1表达含量的变化。采用MTT法、流式细胞术及Transwell法检测BTG1过表达对NSCLC细胞增殖、凋亡及侵袭转移的影响。结果 BTG1蛋白在NSCLC及癌旁正常组织中的阳性表达率分别为37.8%(31/82)、84.2%(32/38),差异有统计学意义(P<0.05)。NSCLC组织中BTG1蛋白的相对表达量为0.331±0.035,明显低于癌旁正常组织的0.673±0.072,差异有统计学意义(P<0.05)。NSCLC组织中BTG1的表达与淋巴结转移、临床分期和组织学分级有关(P<0.05)。BTG1过表达的NSCLC细胞增殖能力明显减弱、细胞凋亡增加及侵袭转移能力降低,且Bcl-2、MMP-9表达量明显下调。结论 NSCLC组织中BTG1蛋白的表达明显降低;BTG1可能通过调控Bcl-2及MMP-9蛋白的表达影响NSCLC细胞的增殖、凋亡与转移。Objective To investigate the expression of B-cell translocation gene 1 （BTG1） in non-small cell lung cancer （NSCLC） and its biological effect in NSCLC cell line by BTG1 overexpression. Methods Immunohistochemistry and Western blotting were used to detect BTG1 protein expression in 82 cases of NSCLC and 38 cases of adjacent normal lung tissues, and the relationship between BTG1 expression and elinicopathological characteristics was analyzed. BTG1 lentiviral vector and empty vector were respectively transfected into NSCLC H1299 cell line. Quantitative real-time RT-PCR（qRT-PCR） and Western blotting were used to de- tect the mRNA and protein level of BTG1. MTT assay, flow cytometry and Transwell assay were also used to detect the effects of the up- regulated expression of BTG1 on H1299 cell proliferation, apoptosis and invasion. Results The positive expression rate of BTG1 pro- tein was 37.8% （31/82） in NSCLC tissues, significantly lower than 84. 2% （32/38） in adjacent normal lung tissues （P〈0 .05 ）. The relative amount of BTG1 protein in NSCLC tissues was 0. 331±0. 035, significantly lower than 0. 673±0. 072 in adjacent normal lung tissues（P〈0.05）. The level of BTG1 protein expression was correlated with lymph node metastasis, clinical stage and histological grade （P〈0. 05）. The result of biological function had shown that H1299 cell transfected BTG1 had a lower survival fraction, higher cell apoptosis, and significant decrease in migration and invasion, and lower Bel-2, MMP-9 protein expression compared with H1299 cell untransfected BTG1 （P〈0. 05）. Conclusion BTG1 expression is decreased in NSCLC tissues, suggesting that BTG1 may play an important role as a negative regnlator to NSCLC H1299 cell by promoting degradation of Bcl-2 and MMP-9 protein.

关 键 词：B细胞易位基因1(BTG1) 非小细胞肺癌 基因转染 肿瘤转移 

分 类 号：R734.2[医药卫生—肿瘤]