Ras相关区域家族1A基因在喉癌及喉角化症组织中的表达及临床意义  

Immunohistochemical Expression and Clinical Implication of RASSF1A Gene in Laryngeal Squamous Cell Carcinoma and Keratosis of the Larynx

作　　者：李万举[1] 孙敬武[1] 别远志[1] 

机构地区：[1]安徽省立医院耳鼻咽喉头颈外科,合肥230001

出　　处：《听力学及言语疾病杂志》2014年第6期616-619,共4页Journal of Audiology and Speech Pathology

摘　　要：目的探讨Ras相关区域家族1A基因(Ras association domain family 1A,RASSF1A)的表达与喉癌发生发展的关系。方法收集喉角化症组织43例、喉鳞癌组织31例作为实验组,取冷冻新鲜尸头正常声带黏膜8例作为正常对照组,用免疫组织化学方法检测各组组织的RASSF1A蛋白表达情况并进行分析比较。结果正常喉黏膜、喉角化症及喉鳞状细胞癌组织中RASSF1A阳性细胞表达率分别为58.25%±8.8%、47.22%±2.25%和21.36%±10.98%,喉鳞状细胞癌组织中RASSF1A的表达强度低于喉角化症及正常喉粘膜组织,差异均有统计学意义(P<0.05),喉角化症与正常喉黏膜组织中的RASSF1A阳性表达率差异无统计学意义(P>0.05)。在I^IV期喉癌组织中RASSF1A的表达强度逐渐降低,差异有统计学意义(P<0.05),而高分化、中分化及低分化癌组织中RASSF1A阳性细胞表达率比较,差异无统计学意义(P>0.05)。结论 RASSFlA表达降低可能与喉鳞状细胞癌的发生发展有关,对鉴别癌前病变与喉癌具有一定意义。Objective To observe the RASSF1A expression in laryngeal keratosis and laryngeal squamous cell carcinoma and investigate the relationship between the gene and the occurrence and development of this disease. Methods The specimens of keratosis of larynx（43 cases）and laryngeal squamous cell carcinoma（31 cases）in Oto-laryngology-Head and Neck surgery of Anhui provincial hospital from Dec 2009 to Dec 2012 were collected.Nor-mal vocal fold mucosa from the cadaveric head as control group（8 cases）were collected.Immunohistochemical meth-ods were used to detect the expression of RASSF1A protein in these tissues.The diffuse distribution of brown gran-ules in the cell cytoplasm yielded positive results and the nucleus was not coloring.The percentage of positive cells of every section,and the average standard deviation（￣x±s）were calculated.SPSS17.0 statistical analysis software was used to analyze the data,P〈0.05 for the difference was statistically significant.ResuIts The RASSF1A expes-sion in laryngeal squamous cell carcinoma,normal laryngeal mucosa,squamous cell carcinoma of the larynx and la-ryngeal keratosis were compared.The differences of RASSF1A expression were statistically significant （P〈0.05）. The difference of RASSF1A expression in keratosis of larynx and normal laryngeal mucosa was not significant （P〉0.05）.RASSF1A expression in the clinical stage from I to IV of laryngeal squamous cell carcinoma gradually de-clined.During the clinical stage,the difference had statistics significance （P〈0.05 ）.In high differentiated squa-mous cell carcinoma,moderately differentiated squamous and poorly differentiated squamous,the differences of RASSF1A＆ expression were insignificant （P〉0.05）.ConcIusion The reduction of RASSF1A expression correlated with laryn-geal squamous cell carcinoma,which has certain significance for the different ion of carcinoma and laryngeal precan-cerous lesion.

关 键 词：喉癌 喉角化症 Ras相关区域家族1A基因 

分 类 号：R739.6[医药卫生—肿瘤]