汉族人载脂蛋白E基因多态性与脑梗死部位及梗死灶体积的相关性研究  

Relationships between apolipoprotein E gene polymorphisms and both location and size of cerebral infarction

作　　者：敬淮淞[1] 周杰[1] 张苓[1] 刘洛同[1] 明扬[1] 陈礼刚 

机构地区：[1]泸州医学院附属医院神经外科,泸州646000

出　　处：《中华神经医学杂志》2014年第12期1248-1251,共4页Chinese Journal of Neuromedicine

摘　　要：目的探讨载脂蛋白E（ApoE）基因多态性与脑梗死发生部位及梗死灶体积的相关性。方法采用病例-对照研究设计，对自2011年9月至2012年11月泸州医学院附属医院神经内、外科收治的162例脑梗死患者（脑梗死组）和同期体检的120例健康人（对照组）的血样采用多重聚合酶连接反应-连接酶检测反应分型法（PCR-LDR）测定ApoE基因的多态性，分析不同脑梗死部位ApoE基因型分布及各等位基因频率的差异，以及各基因型与梗死灶体积的关系。结果脑梗死组和对照组之间ApoE基因型分布和等位基因频率比较差异均无统计学意义（P〉02．05）。各脑梗死部位间ApoE基因型分布和等位基因频率比较差异均无统计学意义（P〉0．05）。脑梗死组中E4／）（基因型（E4／3基因型和E4／4基因型合并）的平均梗死灶体积[（15．7±2．3）cm^3]与E3／3基因型[（10．2±2．1）cm^3]及E2／）（基因型（E2／2基因型和E2／3基因型合并）[（9．5±1．8）cm^3]比较差异具有统计学意义（P〈0．05），且E4／X基因型中大面积脑梗死比例（56％）较E3／3基因型（28．1％）及E2／X基因型（26．3％）更高，差异具有统计学意义（P〈0．05）。结论ApoE基因多态性与脑梗死发生部位无相关性，而与梗死灶体积相关，其中携E4等位基因者较易发生大面积脑梗死。Objective To investigate the relationships between the apolipoprotein E （ApoE） gene polymorphisms and both the location and size of cerebral infarction. Methods A case-control study was chosen; 162 cerebral infarction patients, admitted to our hospital from September 2011 to November 2012, and 120 normal controls were chosen; their blood samples were detected by multiple polymerase chain reaction-ligase detection reaction （PCR-LDR） to analyze the ApoE gene polymorphisms. Its differences of genotype distribution or allele frequencies in different locations of cerebral infarction were analyzed, and the relationships between allele frequencies and volume of cerebral infarction was analyzed. Results No significant difference was found either in ApoE genotype distributions or allele frenquencies between the cerebral infarction patients and controls （P〉0.05）; the ApoE genotype distributions or allele frenquencies showed no significant difference among patients having different locations of cerebral infarction （P〉0.05）. A significant difference was observed in the average infarction volume between E4/X carriers （the E4/3 and E4/4 genotype combination, [15.7±2.3] cm^3） and E3/3 carries （[10.2±2.1] cm^3） and E2/X carriers （the E2/2 and E2/3 genotype combination, [9.5± 1.8] cm^3, P〈 0.05）; and the ratio of massive cerebral infarction of E4/X carriers （56%） was significantly higher than E3/3 or E2/X carriers （26.3%, P〈0.05）. Conclusions There is no correlation between ApoE genotype polymorphisms and cerebral infarction location; however it may be associated with infarction volume. The E4/X carriers are more prone to massive cerebral infarction, and its volume is larger than that of E2 and E3.

关 键 词：载脂蛋白E 脑梗死 基因多态性 多重聚合酶连接反应-连接酶检测反应分型法 

分 类 号：R743.3[医药卫生—神经病学与精神病学]