NS和PUMA蛋白在人脑胶质瘤中的表达及其临床意义  

Expressions of nucleostemin and p53 up-regulated apoptotic factor proteins in human gliomas and clinical significance

作　　者：程森[1] 寿记新[1] 付旭东[1] 马建[1] 周少龙[1] 

机构地区：[1]郑州大学第五附属医院神经外科,郑州450052

出　　处：《中华神经医学杂志》2014年第12期1256-1259,共4页Chinese Journal of Neuromedicine

摘　　要：目的探讨核干因子（NS）和p53上调凋亡因子（PUMA）蛋白在人脑胶质瘤中的表达及其临床意义。方法收集郑州大学第五附属医院神经外科自2011年1月至2013年2月手术切除并经病理证实的脑胶质瘤标本62例，其中WHO分级Ⅰ级8例、Ⅱ级21例，Ⅲ级19例，Ⅳ级14例。另取同期10例因颅脑损伤行内减压术获取的的正常脑组织作为对照组，免疫组化染色检测标本中NS和PUMA蛋白的表达。结果胶质瘤组织NS蛋白的表达明显高于对照组，胶质瘤组织PUMA蛋白的表达明显低于对照组，差异均有统计学意义（P〈0．05）；与Ⅰ＋Ⅱ级胶质瘤比较，Ⅲ＋Ⅳ级胶质瘤组织NS蛋白的表达较高（0．224±0．015vs0．302±0．024），PUMA蛋白的表达较低（0．182±0．020vs0．167±0．0181，差异有统计学意义（P〈0．05）。结论NS和PUMA蛋白与胶质瘤的恶性程度密切相关。二者在胶质瘤的发生和发展过程中起重要的作用。Objective To investigate the expressions ofnucleostemin （NS） and p53 up-regulated apoptotic factor （PUMA） proteins in human gliomas and its clinical significance. Methods Sixty-two human gliomas, collected in our hospital from January 2011 to February 2013 and confirmed by pathology, were used in our study; according to the WHO grading, grade Ⅰ included 8 patients in the study group, grade Ⅱ 21 patients, grade Ⅲ 19 patients and grade Ⅳ 14 patients. Other 10 patients accepted decompressions in our hospital at the same time period were used as control group. The expressions of NS and PUMA proteins in the brain tissues were detected by immunohistochemical S-P method. Results The NS protein expression was significantly higher and PUMA protein expression was statistically lower in study group than those in the control group （P〈0.05）. As compared with gliomas of grade Ⅰ and Ⅱ, gliomas of grade Ⅲ and Ⅳ had significantly higher NS protein expression and lower PUMA protein expression （P〈0.05）. Conclusions The expression changes of NS and PUMA are closely associated with gliomas malignant degrees, and the two play important roles in the progression and proliferation ofglioma cells.

关 键 词：核干因子 p53上调凋亡因子 神经胶质瘤 免疫组化 

分 类 号：R739.41[医药卫生—肿瘤]