鳖甲煎丸对Wnt信号通路中β-catenin/TCF4复合物活性及信号分子cyclin D1、MMP-2的影响  

Effects of Biejiajian Pills on Wnt signal pathway signal molecules β-catenin/TCF4 complex activities and downstream proteins cyclin D1 and MMP-2 in hepatocellular carcinoma cells

作　　者：文彬[1] 孙海涛[2] 贺松其[2] 程旸[3] 贾文燕[2] 樊尔艳 庞杰[2] 

机构地区：[1]中国人民解放军第四五八医院中医科,广东广州510602 [2]南方医科大学中医药学院,广东广州510515 [3]南方医科大学第一临床学院,广东广州510515

出　　处：《南方医科大学学报》2014年第12期1758-1762,共5页Journal of Southern Medical University

基　　金：国家自然科学基金(81072934);广东省自然科学基金(9151051501000003)~~

摘　　要：目的探讨鳖甲煎丸抗肝细胞癌转移侵袭的分子机制。方法使用含鳖甲煎丸药物血清培养肝癌细胞Hep G2,48 h后采用免疫荧光技术检测Wnt/β-catenin通路信号分子β-catenin蛋白表达水平,双荧光素酶检测法检测β-catenin/TCF4复合物的活性,采用RT-PCR技术检测cyclin D1与MMP-2基因转录水平,Western blot技术检测cyclin D1与MMP-2的蛋白表达水平。结果含鳖甲煎丸药物血清可降低肝癌细胞Hep G2细胞核中β-catenin蛋白的表达水平,抑制β-catenin/TCF4复合物的活性,明显下调cyclin D1、MMP-2的基因转录及其蛋白的表达水平。结论鳖甲煎丸可抑制Wnt/β-catenin信号通路,从而抑制肝癌细胞Hep G2的生长、粘附和转移。Objective To study the effect of Biejiajian Pills on Wnt signal pathway and the mechanisms underlying its action to suppress the invasiveness of hepatocellular carcinoma. Methods HepG2 cells cultured in the serum of rats fed with Biejiajian Pills for 48 h were examined for β-catenin expression using immunofluorescence, β-catenin/TCF4 complex activity with luciferase, and expressions of the downstream proteins cyclin D1 and MMP-2 using qRT-PCR. Results Biejiajian Pills-treated sera significantly reduced the expressions of cytoplasmic and nuclear β-catenin protein, cyclin D1 and MMP-2 proteins and lowered the activities ofβ-catenin/TCF4 complex. Conclusion Biejiajian Pills may serve as a potential anti-tumor agent, whose effect might be mediated by inhibiting the Wnt/β-catenin pathway.

关 键 词：肝癌细胞 鳖甲煎丸 β-catenin/TCF4复合物 CYCLIN D1 MMP-2 CYCLIN D1 MMP-2 

分 类 号：R285[医药卫生—中药学]