外周血及大肠癌组织TGF-β1含量检测及临床意义  

Detection of transforming growth factor-β1 in colorectal cancer and its clinical significance

作　　者：黄永红[1] 曹云飞[1] 高枫[1] 张森[1] 张磊昌[1] 龙军先[1] 

机构地区：[1]广西医科大学第一附属医院结直肠肛门外科,广西南宁530021

出　　处：《南方医科大学学报》2014年第12期1790-1793,共4页Journal of Southern Medical University

基　　金：国家自然科学基金(81260316)~~

摘　　要：目的探讨转化生长因子(TGF-β1)在结直肠癌患者的表达情况及临床意义。方法采用ELISA方法检测50例结直肠癌患者术前、术后,以及25例健康志愿者的外周血TGF-β1表达水平差异,同时检测了结直肠癌肿瘤组织和癌旁正常大肠组织匀浆上清的TGF-β1含量,分析TGF-β1浓度的变化与临床病理指标的关系。结果结直肠癌患者术前血浆TGF-β1水平明显高于术后组以及健康对照组,差异有统计学意义(P<0.05);肿瘤组织TGF-β1含量显著高于癌旁正常大肠组织(P<0.05);在CEA阴性及CEA阳性患者中均可分别观察到类似的浓度改变。外周血TGF-β1水平与肿瘤大小呈正相关,随着肿瘤分期的进展,浓度明显升高。结论 TGF-β1可联合CEA检测,更好的为结直肠癌的临床诊断、评价预后和复发提供参考价值,同时为基于抗TGF-β1的肿瘤免疫治疗提供参考依据。Objective To investigate the role of transforming growth factor β1 （TGF-β1） in patients with colorectal cancer. Methods Fresh peripheral blood were obtained from 50 patients （before surgery and at least one week after surgery） and 25 healthy donors in the morning. Fresh colorectal cancer tissues and the adjacent tissues （at least 5 cm from the tumor site） were obtained from patients undergoing tumor resection. The expression levels of TGF-β1 in the blood and tissue specimens were determined using ELISA. Results The plasma levels of TGF-β1 in patients with colorectal cancer were significantly higher than those in the healthy donors, and decreased after the surgery （P〈0.05）. The tumor tissues expressed higher levels of TGF-β1 than the adjacent tissues from both CEA-negative and-positive patients. The plasma level of TGF-β1 in the patients were positively correlated with the tumor size and clinical tumor stage （P〈0.05）. Conclusion TGF-β1 combined with CEA can provide important information for the diagnosis, prognostic assessment and prediction of recurrence in patients with colorectal cancer, and may provide new insights for anti-TGF-β1-based tumor immune therapeutic strategies.

关 键 词：TGF-Β1 结直肠癌 诊断 免疫治疗 TRANSFORMING GROWTH factorβ1 

分 类 号：R735.34[医药卫生—肿瘤]