蜂毒肽对前列腺癌PC-3细胞凋亡诱导的影响  

Effects of Melittin on apoptosis of prostate cancer cell PC-3

作　　者：吕立国[1] 白遵光[1] 张娴[2] 吴巧玲[3] 陈志强[1] 王昭辉[1] 代睿欣[1] 王树声[1] 

机构地区：[1]广东省中医院泌尿外科,广州510120 [2]广东省中医院中心实验室 [3]广东省中医院肿瘤科

出　　处：《中国男科学杂志》2014年第9期13-16,共4页Chinese Journal of Andrology

基　　金：广东省科技计划项目（2012B061700035）

摘　　要：目的：研究蜂毒肽（Melittin）对人前列腺癌PC-3细胞凋亡的影响及可能机制。方法实验设立空白对照组和蜂毒肽不同浓度组，用MTT方法检测细胞增殖，Hoechst 33258染色检测细胞凋亡，RT-PCR检测p27、p53 mRNA表达，Western blot检测蛋白表达。结果与对照组比较，蜂毒肽作用24、48、72 h后，4、8、16、32μg/mL组MTT检测OD值明显降低，细胞凋亡率明显增高（P＜0.05），作用48h后，p27、p53 mRNA表达明显升高（P＜0.05），p53和 Caspase3蛋白含量增高。结论蜂毒肽对PC-3细胞有凋亡诱导作用，其作用机制可能与增高p27、p53、Caspase3表达相关。Objective To study the effects of Melittin （Mel） on apoptosis of prostate cancer cell PC-3 and explore its potential mechanism. Methods Blank control Group and Mel groups of different concentrations were set up in this study. Cell proliferation was analyzed by MTT assay. Cell apoptosis was measured by Hoechst 33258 dying method. The expressions of p27and p53were detected by RT-qPCR, and their protein expressions were detected by Western blot. Results Compared with those of the controls, cell proliferation （OD value） decreased and apoptosis increased significantly in Mel groups of 4, 8, 16, 32 μg/mL after 24, 48 and 72 h treatment. After 48h treatment, the expressions of p27 andp53 mRNA were increased significantly, and protein expression of p53 and caspase3 were also increased. Conclusion Mel may induce PC-3 cell apoptosisby upregulation of p27, p53 and caspase3 expression.

关 键 词：蜂毒肽 前列腺肿瘤 细胞系  肿瘤 细胞凋亡 

分 类 号：R737.25[医药卫生—肿瘤]