Methanolic extract of Momordica cymbalaria enhances glucose uptake in L6 myotubes in vitro by up-regulating PPAR-γ and GLUT-4  

作　　者：Puttanarasaiah Mahesh Kumar Marikunte V Venkataranganna Kirangadur Manjunath Gollapalle L Viswanatha Godavarthi Ashok 

机构地区：[1]Padmashree Institute of Information Sciences [2]Connexios Life Sciences Pvt Ltd.,J.P.Nagar, Bangalore 560078, India [3]Department of Microbiology and Biotechnology, Bangalore University [4]PES College of Pharmacy [5]Radiant Research Services Pvt Ltd.

出　　处：《Chinese Journal of Natural Medicines》2014年第12期895-900,共6页中国天然药物（英文版）

基　　金：supported by Padmashree Institute of Information Sciences,Bangalore

摘　　要：The present study was undertaken to evaluate the influence of the methanolic fruit extract of Momordica cymbalaria （MFMC） on PPARγ, （Peroxisome Proliferator Activated Receptor gamma） and GLUT-4 （Glucose transporter-4） with respect to glucose transport. Various concentrations of MFMC ranging from 62.5 to 500 μg·mL^-1 were evaluated for glucose uptake activity in vitro using L6 myotubes, rosiglitazone was used as a reference standard. The MFMC showed significant and dose-dependent increase in glucose uptake at the tested concentrations, further, the glucose uptake activity of MFMC （500 μg·mL^-1） was comparable with rosigilitazone. Furthermore, MFMC has shown up-regulation of GLUT-4 and PPARy gene expressions in L6 myotubes. In addition, the MFMC when incubated along with cycloheximide （CHX）, which is a protein synthesis inhibitor, has shown complete blockade of glucose uptake. This indicates that new protein synthesis is required for increased GLUT-4 translocation. In conclusion, these findings suggest that MFMC is enhancing the glucose uptake significantly and dose dependently through the enhanced expression of PPARμ and GLUT-4 in vitro.The present study was undertaken to evaluate the influence of the methanolic fruit extract of Momordica cymbalaria(MFMC) on PPARγ(Peroxisome Proliferator Activated Receptor gamma) and GLUT-4(Glucose transporter-4) with respect to glucose transport. Various concentrations of MFMC ranging from 62.5 to 500 μg·mL-1 were evaluated for glucose uptake activity in vitro using L6 myotubes, rosiglitazone was used as a reference standard. The MFMC showed significant and dose-dependent increase in glucose uptake at the tested concentrations, further, the glucose uptake activity of MFMC(500 μg·mL-1) was comparable with rosigilitazone. Furthermore, MFMC has shown up-regulation of GLUT-4 and PPARγ gene expressions in L6 myotubes. In addition, the MFMC when incubated along with cycloheximide(CHX), which is a protein synthesis inhibitor, has shown complete blockade of glucose uptake. This indicates that new protein synthesis is required for increased GLUT-4 translocation. In conclusion, these findings suggest that MFMC is enhancing the glucose uptake significantly and dose dependently through the enhanced expression of PPARγ and GLUT-4 in vitro.

关 键 词：Momordica cymbalaria DIABETES Glucose transport PPARY L6 myotubes Antidiabetic activity 

分 类 号：R965[医药卫生—药理学]