1株HoBi样瘟病毒的分离与序列分析  

Isolation and sequence analysis of a HoBi-like pestivirus

作　　者：毛立[1] 李文良[1] 张纹纹[1] 杨蕾蕾[1] 刘霞[1] 江杰元[1] 

机构地区：[1]江苏省农业科学院兽医研究所/农业部兽用生物制品工程技术重点实验室/国家兽用生物制品工程技术研究中心,江苏南京210014

出　　处：《中国兽医学报》2014年第5期717-721,共5页Chinese Journal of Veterinary Science

基　　金：江苏省农业科技自主创新资金资助项目(SCX(12)3143)

摘　　要：本研究在MDBK细胞培养物中发现并分离出1株HoBi样瘟病毒,将该毒株命名为JS12/01。应用RT-PCR方法分段扩增该毒株的全基因组序列,并对其基因组序列进行分析。结果显示,该毒株基因序列2端分别为5′端非编码区(5′untranslated region,5′-UTR)和3′端非编码区(3′untranslated region,3′-UTR),编码区为11 700nt。与GenBank中登录的部分瘟病毒属代表毒株5′-UTR区序列进行进化分析,结果表明,该毒株属于HoBi样瘟病毒巴西源亚型。本研究中的HoBi样瘟病毒JS12/01株为国内首次分离鉴定,证实了该病毒在中国的存在,需要对该病毒的扩散与流行采取预防措施。HoBi-like pestivirus, which was first isolated in 2004, belongs to family Flaviviridae, genus Pestivirus. In this study,a novel HoBi-like pestivirus named JS12/01 was detected by RT- PCR in contaminated MDBK cells that were used to isolate the samples of suspect bovine viral diarrhea virus infections. The near-full length genome sequence of the virus strain was cloned and sequenced. The results indicated that the genome comprises an open reading frame of 11 700 nu- cleotides （nt） flanked by 5′and 3′-untranslated regions （UTRs）. The 5′-UTR sequence was analyzed with some reference sequences of pestiviruses in Genbank. Phylogenetic analysis demonstra- ted it was more strictly related to the Hobi-like viruses from Brazil than Thai origin. It is the first report of HoBi-like pestivirus isolated in China,and the information is important for surveillance and prevention of this emerging animal infectious disease.

关 键 词：HoBi样瘟病毒 全基因组 序列分析 

分 类 号：S852.65[农业科学—基础兽医学]