己烯雌酚降解菌株沙雷氏菌的分离鉴定及其降解特性  

Isolation,Identification and Characterization of a Diethylstilbestrol-degrading Bacterial Strain Serratia sp.

作　　者：徐冉芳 孙敏霞[1] 刘娟[1] 汪泓[1] 李欣[1] 朱雪竹[1] 凌婉婷[1] 

机构地区：[1]南京农业大学土壤有机污染控制与修复研究所,南京210095

出　　处：《环境科学》2014年第8期3169-3174,共6页Environmental Science

基　　金：江苏省科技支撑计划项目(BE2011780);国家自然科学基金项目(51278252;41171193)

摘　　要：利用具有降解己烯雌酚(diethylstilbestrol,DES)特性的功能微生物来降解DES、有望实现环境中DES的有效去除,然而迄今关于降解DES的功能菌株及其降解特性的报道很少.本研究通过选择性富集培养,从某污水处理站活性污泥中分离获得1株具有DES降解特性的细菌(菌株S).经形态观察、生理生化试验及16S rDNA序列同源性分析将菌株S鉴定为沙雷氏菌属(Serratia sp.)细菌.菌株S好氧生长,30℃、150 r·min-1摇床培养7 d后,对无机盐培养基中DES(50 mg·L-1)的降解率达68.3%.通过摇瓶实验,优化了菌株S生长和降解DES的最适环境条件:温度30℃,底物浓度40～60 mg·L-1,pH 7.0,接种量5%,盐添加量0 g·L-1,装液量10 mL(100 mL三角瓶).Utilizing the diethylstilbestrol（DES）-degrading bacteria to biodegrade DES is a most reliable technique for cleanup of DES pollutants from the environment. However,little information is available heretofore on the isolation of DES-degrading bacteria and their DES removal performance in the environment. A novel bacterium capable of degrading DES was isolated from the activated sludge of a wastewater treatment plant. According to its morphology,physiochemical characteristics,and 16 S rDNA sequence analysis,this strain was identified as Serratia sp.. The strain was an aerobic bacterium,and it could degrade 68. 3% of DES（50 mg·L^-1） after culturing for 7 days at 30℃,150 r·min^-1 in shaking flasks. The optimal conditions for DES biodegradation by the obtained strain were 30℃,40-60 mg·L^-1DES,pH 7. 0,5% of inoculation volume,0 g·L^-1of added NaCl,and 10 mL of liquid medium volume in 100 mL flask.

关 键 词：己烯雌酚 雌激素 菌株鉴定 生物降解 沙雷氏菌属 

分 类 号：X172[环境科学与工程—环境科学]