根瘤菌Sneb183对大豆胞囊线虫二龄幼虫的作用方式研究  

Action Mode of Sinorhizobium fredii Sneb183 on the Activity of Soybean Cyst Nematode

作　　者：田丰[1] 陈立杰[1] 王媛媛[2] 朱晓峰[1] 段玉玺[1] 

机构地区：[1]沈阳农业大学植物保护学院,沈阳110866 [2]沈阳农业大学生物技术学院,沈阳110866

出　　处：《中国生物防治学报》2014年第4期540-545,共6页Chinese Journal of Biological Control

基　　金：公益性行业(农业)科研专项(200903040-03);现代农业产业技术体系(CARS-04-PS13)

摘　　要：大豆胞囊线虫是大豆生产中的主要病害之一,由于化学防治存在费用高,效果不佳,污染环境和危害人畜安全等方面的诸多问题,生物防治成为了防治大豆胞囊线虫的新方法。本试验利用体外生物测定方法,研究了费氏中华根瘤菌Sinorhizobium fredii Sneb183对大豆胞囊线虫二龄幼虫的存活、卵孵化、呼吸作用以及体液渗透的影响。结果表明,根瘤菌Sneb183处理幼虫72 h后,死亡率达86.7%,处理初期,线虫出现活动迟缓等症状,而后表现出整个身体僵直的死亡症状;对卵的孵化抑制率可达59.4%;根瘤菌Sneb183对二龄幼虫呼吸有较强抑制作用,处理24 h后,耗氧量下降93.1%;同时根瘤菌Sneb183发酵液可引起二龄幼虫体液渗漏,处理48 h后电导率增加了62.4%。因此,根瘤菌Sneb183可成为大豆胞囊线虫病生物防治资源中的新成员。The soybean cyst nematode （SCN,Heterodera glycines） is one of the most important pests in soybean production. Due to high economic and environmental costs associated with chemical control of nematode, endophytic rhizobacteria have been used successfully for the biological control of SCN. This paper measured mortality, egg hatching, oxygen consumption and permeability of body fluids of SCN second-stage juvenile which was treated by fermentation liquor of Sneb183 （Sinorhizobium fredii）. When treated to nematode juvenile, Sneb183 resulted in 86.7% of nematode juvenile at 72 h post-treatment. Sneb183 inhibited nematode hatching by 59.4%. At 24 h after treatment, the oxygen consumption rate was decreased by 93.1%, while at 48 h after treatment, the conductivity was increased by 62.4%. The study suggested that Sneb183 could be a new agent for biological control of soybean cyst nematode.

关 键 词：大豆胞囊线虫 费氏中华根瘤菌Sneb183 作用方式 发酵液 

分 类 号：S476[农业科学—农业昆虫与害虫防治]