人肾上腺皮质癌裸鼠皮下移植瘤模型的建立  

Establishment of Subcutaneous Xenograft Model of Human Adrenocortical Carcinoma in Nude Mice

作　　者：徐文清[1] 肖戈[1] 吴义高[1] 黄福[1] 王尉[1] 胡卫列[1] 

机构地区：[1]广州军区广州总医院泌尿外科,510010

出　　处：《实用癌症杂志》2014年第5期489-490,494,共3页The Practical Journal of Cancer

基　　金：国家自然科学基金项目(81172421);国家自然科学基金青年科学基金资助项目(81001142)

摘　　要：目的建立人肾上腺皮质癌裸鼠皮下移植瘤模型。方法采用人肾上腺皮质癌细胞株SW-13细胞接种于裸鼠腋下皮下,记录成瘤潜伏期,观察肿瘤生长情况,并绘制生长曲线,对移植瘤进行组织病理学鉴定。结果采用SW-13细胞皮下接种方式建立移植瘤的模型成瘤百分率为95%,成瘤平均潜伏期为(18±1.2)d,移植瘤体积倍增时间为(10±1.8)d天,移植瘤组织病理学形态与人肾上腺皮质腺癌相似。结论成功建立人肾上腺皮质癌裸鼠皮下移植瘤模型,为研究其发病机制及诊治方法提供实验依据。Objective To establish a subcutaneous xenograft model of human adrenocortical carcinoma in nude mice . Methods Human adrenocortical carcinoma cell line SW-13 cells were inoculated subcutaneously into the oxter of nude mice . The proliferation of tumor were observed and the growth curve were draw .The pathological identification of tumors were carried out.Results The mean incubation period of xenograft by inoculating subcutaneously with SW -13 cell was （18 ±1.2） days with 95%of tumor forming rate.The time tumor took to double its volume was （10 ±1.8） days.Morphologically, xenograft was simi-lar with the original tumor .Conclusion The establishment of xenograft model of human adrenocortical carcinoma can provide ex -perimental foundation for the study of adrenocortical carcinoma .

关 键 词：肾上腺皮质癌 裸鼠 移植瘤 SW-13细胞株 

分 类 号：R73-35[医药卫生—肿瘤]