弥漫大B细胞淋巴瘤中预后蛋白的表达及bcl-6基因重排情况分析  

Expression of Prognostic Protein and Rearrangement of Bcl-6 Gene in Diffuse Large B-cell Lymphoma

作　　者：黄晖[1] 黄传生[1] 杨文萍[2] 黄慧[2] 

机构地区：[1]江西省肿瘤医院,330029 [2]江西省儿童医院,330006

出　　处：《实用癌症杂志》2014年第5期512-515,共4页The Practical Journal of Cancer

摘　　要：目的探讨弥漫大B细胞淋巴瘤(DLBCL)中预后蛋白的表达及bcl-6基因重排情况。方法应用免疫组化SP法,检测CD5、CD10、bcl-6、MUM1和bcl-2蛋白在29例DLBCL中的表达。根据CD10、bcl-6和MUM1的表达情况将DLBCL分为生发中心B细胞型(GCB型)和非生发中心B细胞型(non-GCB型)。应用荧光原位杂交(FISH)技术检测bcl-6基因重排。结果 29例DLBCL中年龄<18岁者11例,CD5、CD10、bcl-6、MUM1和bcl-2阳性表达率分别为18.2%(2/11)、45.5%(5/11)、54.5%(6/11)、36.4%(4/11)和63.6%(7/11),GCB型6例(54.5%)、non-GCB型5例(45.5%),bcl-6基因断裂1例(10.00%)、扩增2例(20.00%);≥18岁者18例,CD5、CD10、bcl-6、MUM1和bcl-2阳性表达率分别为16.7%(3/18)、0(0/18)、44.4%(8/18)、83.3%(15/18)和55.6%(10/18);GCB型1例(5.6%)、non-GCB型17例(94.4%),bcl-6基因断裂5例(27.8%)。bcl-6和bcl-2蛋白表达儿童DLBCL与成人DLBCL比较差异无统计学意义(P=0.710、0.717)。bcl-2蛋白表达儿童GCB型和non-GCB型DLBCL比较差异无统计学意义(P=1.000)。结论成人DLBCL以non-GCB型为主,bcl-6基因重排常见。儿童DLBCL以GCB型多见,bcl-2蛋白不是DLBCL的预后指标。Objective To investigate the expression of prognostic protein and rearrangement of bcl -6 gene in diffuse large B-cell lymphoma （DLBCL）.Methods Expression of CD5,CD10,bcl-6,MUM-1 and bcl-2 in 29 cases of DLBCL was de-tected by immunohistochemistry SP method .The patients were divided into germinal center B-cell（ GCB） type and non-GCB type according to the expression of CD10,bcl-6 and MUM-1.Fluorescence in situ hybridization（FISH）was employed to detect the rear-rangement of bcl-6 gene.Results 11 patients were under 18 among the 29 patients,in which the positive expression rates of CD5,CD10,Bcl-6,MUM-1 and Bcl-2 were 18.2%（2/11）,45.5%（5/11）,54.5%（6/11）,36.4%（4/11） and 63.7%（7/11）,respectively,and there had 6 cases （54.5%）of GCB type and 5 cases（45.5%）of non-GCB type,bcl-6 gene breakage were founded in 1 cases（10.0%）and bcl-6 gene amplification were detected in 2 cases（20.0%）.18 patients were 18 years old or o-ver 18,the positive expression rates of CD5、CD10、bcl-6、MUM-1 and bcl-2 were 16.7%（3/18）,0（0/18）,44.4%（8/18）,83. 3%（15/18）and 55.6%（10/18）,respectively;there had 1 case （5.6%）of GCB type and 17 cases （94.4%）of non-GCB type, bcl-6 gene breakage were detected in 5 cases（27.8%）.There were no significant difference for bcl-2 an bcl-6 protein expression between children and adult DLBCL patients （P=0.710,0.717）.There were no significant difference for bcl-2 protein expression between children GCB type and none-GCB type.Conclusion Non-GCB is the main type in adult DLBCL patients ,and bcl-6 gene rearrangement is common .GCB type is more common in children DLBCL patients ,and the positive expression of bcl-2 pro-tein is not associated with prognosis of DLBCL .

关 键 词：弥漫大B细胞淋巴瘤 BCL-6基因 荧光原位杂交 

分 类 号：R733.4[医药卫生—肿瘤]