胃癌细胞及临床标本特异结合的多肽的筛选与鉴定  

The selection and characterization of the peptides binding to the cells and tissues of gastric carcinoma

作　　者：李蕊[1] 王瀚[1] 马彩霞[1] 郭永娥 王琦轩 胥谨慧[1] 吴琼[1] 许楠[1] 侯颖春[1] 

机构地区：[1]陕西师范大学生命科学学院,陕西西安710119

出　　处：《陕西师范大学学报（自然科学版）》2014年第4期65-68,共4页Journal of Shaanxi Normal University：Natural Science Edition

基　　金：陕西省自然科学基金资助项目(SF2009178)

摘　　要：从噬菌体随机十二肽库筛选出两个与人胃腺癌细胞SGC-7901表面特异结合的阳性噬菌体克隆,用细胞免疫荧光法鉴定这些克隆与SGC-7901结合的特异性,据亲和力确定克隆GSP5为最佳克隆,进一步以免疫细胞/组织化学等方法鉴定此克隆与胃癌细胞和临床组织的特异性/敏感性.结果显示,噬菌体克隆GSP5对SGC-7901细胞及胃癌临床组织具有较好的结合特异性/敏感性.该多肽有望成为胃癌分子影像诊断与靶向治疗的候选多肽导向分子.Using phage displayed peptides library screening method,we previously screened out two peptides specifically binding to gastric carcinoma cell SGC-7901.The specific affinity of GSP5 clone was confirmed as best using immunofluorescence assays.Then,the specific affinities of GSP5 clone to the cells and tissues of gastric cancer were further confirmed using different immunofluorescence assays under different microscopes.The results show that the peptide clone GSP5 binds to the cells and clinical tissues of gastric cancer at high affinity.Therefore,the clone GSP5 is of the potential to be used as a peptide probe for the early diagnosis and targeting therapy of gastric cancer.

关 键 词：多肽探针 多肽筛选 胃癌 噬菌体肽库 

分 类 号：Q7[生物学—分子生物学] R735.2[医药卫生—肿瘤]