共培养技术及化学絮凝法对小球藻C.vulgaris XJB絮凝的影响  

Effects of Co-culture and Chemical Flocculation Method on C.vulgaris XJB Flocculation

作　　者：周琳[1] 刘龙[1] 谢丽[1] 王思雨[1] 贺莹莹[1] 徐小琳[1] 王长海[2] 

机构地区：[1]石河子大学化学化工学院/新疆兵团化工绿色过程重点实验室/省部共建国家重点实验室培育基地,石河子832003 [2]南京农业大学资源与环境科学学院,南京210095

出　　处：《石河子大学学报（自然科学版）》2014年第5期627-634,共8页Journal of Shihezi University(Natural Science)

基　　金：国家科技支撑计划课题(2011BAD14B01)

摘　　要：微藻是生物柴油理想的原料,但微藻尺寸较小,细胞密度较低,导致微藻采收困难。为提高微藻的采收率,节约成本,实验采用自絮凝斜生栅藻Scenedesmus sp.-BH与富油小球藻C.vulgaris XJB共培养技术来提高小球藻C.vulgaris XJB的絮凝率,同时与调节p H、添加Fe Cl3和添加壳聚糖3种化学絮凝法对比。结果表明:3种化学絮凝法60 min时的最佳絮凝率分别为84.2%、96.5%和72.9%;共培养最佳条件下小球藻C.vulgaris XJB 60 min时的絮凝率为33.3%,8 h后的絮凝率可达73.2%。共培养条件下小球藻C.vulgaris XJB的絮凝率比化学絮凝法的稍低,但该方法不会引入有害化学物质,后期分离成本低,是一种经济、环保的絮凝方法。Microalgae are ideal raw materials of biodiesel production.However,their microscopic sizes and low cell density result in the difficulty of collection.In order to improve the recovery efficiency of microalgae and obtain a potentially efficient flocculation method,this work focused on co-cuhuring self-flocculating microalgae Scenedesmus sp.-BH and oil-rich microalgae C.vulgaris XJB.Moreover,adjusting pH,adding FeC13 or chitosan were tested in this work.Results showed that,the best flocculating rate for chemical floeculation method after 60 minutes were 84.2%,96.5% and 72.9%,respectively.The best flocculating rate of co-culture was 33.3% after 60 minutes,and the flocculating rate could reach 73.2% in 8 h.The C.vulgaris XJB＇s flocculating rate with co-culture method was lower than chemical flocculation method,but this method didn＇t introduce extra harmful chemicals into the product. It could be an economy and environmentally friendly flocculation method.

关 键 词：絮凝 小球藻C.vulgaris 共培养 油脂含量 

分 类 号：Q949.2[生物学—植物学] S968.41[农业科学—水产养殖]