应用PCR-DGGE技术研究石莼、网地藻藻际微生物多样性  

Preliminary Application of PCR-DGGE to Analyzing Microbial Diversity of Ulva lactuca L. and Dictyota dichotoma

作　　者：冯学珍[1] 伍善广[1] 陆苑[1] 

机构地区：[1]广西科技大学,柳州545006

出　　处：《生物技术通报》2014年第12期73-77,共5页Biotechnology Bulletin

基　　金：广西壮族自治区教育厅级课题(2013LX180);广西高等学校特色专业及课程一体化建设项目(药学GXTSZY286)

摘　　要：应用变性凝胶梯度电泳(PCR-DGGE)技术对石莼、网地藻藻际微生物多样性进行研究,并对其进行相似性、未加权聚类分析(UPGMA)及微生物多样性(Shannon)指数分析。结果表明,PCR-DGGE图谱显示,石莼、网地藻藻际微生物DGGE图谱有着明显的差异性,具体表现在条带数及密度值的不同。Quantity one图谱分析表明:石莼藻际微生物16S r RNA基因的V3区共分离得到25条DNA片段,网地藻为16条DNA片段。DGGE相似性及未加权聚类分析表明:网地藻藻际微生物间的相似性为77.78%,石莼藻际微生物细菌群落间的相似性为49.25%。Shannon指数分析表明,石莼藻际微生物多样性指数显著高于网地藻(P<0.05)。石莼藻际微生物细菌群落较网地藻丰富。To study the microbial diversity of Ulva lactuca L. and Dictyota dichotoma. As a new DNA fingerprinting technique, denaturing gradient gel electrophorcsis （ DGGE ） can be used to analyze the microbial diversity in different environmental samples. Ulva lactuca L. and Dictyota dichotoma will be studied with respect to microbial diversity by applying cultivation-independent molecular methods DGGE. The results showed, the profile of DGGE showed that Ulva lactuca L. and Dictyota dichotoma had different bands. The structural diversity of the microbial community was examined by the Shannon index of general diversity H. Quantity one analysis showed that, V3 region of 16S rRNA gene was isolated from 25 DNA fragments of Ulva lactuca L. and 16 of Dictyota dichotoma. DGGE similarity and UPGMA analysis showed that ： the similarity of Ulva lactuca L. was higher than the Dictyota dichotoma （ P〈0.05 ）.. Shannon indexes of two kinds of algae all showed Ulva lactuca L, wero significantly higher than the Dictyota dichotoma （ P〈0.05 ） . The algae microbial diversity of Ulva lactuca L. was richer than Dictyota dichotoma,

关 键 词：变形凝胶梯度电泳(PCR-DGGE) 石莼 网地藻 藻际微生物 微生物多样性 

分 类 号：Q949.2[生物学—植物学] Q938