链霉素抗性突变理性筛选新霉素高产菌株  

Streptomycin-resistant Rational Screening for High-yield Neomycin Producing Strain

作　　者：陈宏[1] 张祝兰[1] 孙菲[1] 张引[1] 周璟明[1] 郑榕[1] 

机构地区：[1]福建省微生物研究所福建省新药(微生物)筛选重点实验室,福州350007

出　　处：《生物技术通报》2014年第12期173-176,共4页Biotechnology Bulletin

基　　金：国家"十一五""重大新药"创制项目(2010ZX09401-403);福建省自然科学基金项目(2012J01094)

摘　　要：采用化学诱变剂NTG结合链霉素抗性筛选法获得新霉素高产菌株。出发菌株费氏链霉菌(Streptomyces fradiae)FS1109的孢子悬液经不同剂量的化学诱变剂NTG处理后,涂布在含链霉素最小抑制浓度(3μg/m L)的培养基平板上培养,获得大量的链霉素抗性突变株。经影印法初筛和摇瓶发酵复筛,正突变率高于负突变率,获得一株遗传性状稳定的Streptomyces fradiae Str 63菌株,其新霉素生物活性单位比出发菌株提高了50%以上,且C组分较出发菌株的低。NTG mutagenesis and streptomycin resistance screening was applied to screen neomycin high producing strain. A number of streptomycin-resistant （str） mutants were obtained from original strain of Streptomyces fradiae FS1109 treated with three different dosage of NTG under the selection pressure of resistance to streptomycin （ the lethal concentration is 3 gg/mL ） . Streptomycin-resistant mutants strain Str63 was obtained with high neomycin yield by replica plating method and the neomycin productivity could be more than 50% higher and the component of C was lower than that of the original strain in the rotation-flask experiments, the rate of the positive mutation was larger than that of the negative mutation.

关 键 词：费氏链霉菌 新霉素 链霉素抗性筛选 突变 

分 类 号：Q93[生物学—微生物学]