三种鳜鱼骨骼肌生长及相关调控基因表达比较  

Comparison on Muscle Growth and Expression of Muscle Growthrelated Genes Among Three Mandarin Fishes

作　　者：许淼洋[1] 赵金良[1] 李传阳[1] 钱叶周 吴超 钱德 

机构地区：[1]上海海洋大学农业部淡水水产种质资源重点实验室,上海201306 [2]池州市秋浦特种水产开发有限公司,池州247104

出　　处：《生物技术通报》2014年第12期184-189,共6页Biotechnology Bulletin

基　　金：池州市秋浦特种水产开发有限公司专项(2012A01)

摘　　要：通过制作骨骼肌石蜡切片研究了鳜、斑鳜及斑鳜(♀)×鳜(♂)杂交一代不同发育时期(孵化后10D、20D、30D、60D、90D)骨骼肌肌纤维的生长特征,同时,利用q PCR技术分析了肌肉中myostatin、Myo D、myogenin三个基因m RNA的表达变化。结果表明,3种鳜鱼全长均随日龄呈增大趋势,种间生长差异显著,鳜生长最快,斑鳜最慢,杂交一代介于斑鳜和鳜之间。不同发育时期,3种鳜鱼骨骼肌肌纤维数目较为接近,增生生长种间无明显差异,而肌纤维平均直径大小与其生长快慢间呈正相关,表明不同鳜鱼种间生长差异主要由肌肉肥大生长引起的。鳜、斑鳜和杂交一代myostatin m RNA在D20出现表达高峰,之后表达量呈下降趋势。Myo D在D10、D20表达水平较高,后期表达水平下降;Myogenin在D20时表达量最低,之后,鳜表达量升高最为明显,杂交一代次之,斑鳜保持平稳。不同种类鳜鱼肌肉生长差异与3种基因表达差异间存在一定相关性。Muscle samples of Siniperca chuatsi ♂×S. scherzeri ♀ F1 and their parents at five developmental stages in 90 days after hatching. The skeletal muscle growth characteristics were identified by numbers and diameters of muscle fibers through paraffin sections. Meantime were obtained and the mRNA expression of myostatin, MyoD, myogenin in muscle was detected by quantitative PCR. The results showed there were significant differences in growth among three mandarin fish. Siniperca chautsi grew the fastest, F1 secondly and S. scherzeri kept the last. At different developmental stages, numbers of muscle fibers in three mandarin fish showed no significant difference, while the average diameters of the muscle fibers showed positive correlation with growth rate. Growth differences among three mandarin were mainly caused by muscle hypertrophy. The myostatin mRNA expression peak appeared in D20, then the expression showed a downward trend. MyoD mRNA expressed highly in D10, D20 and decreased in following days. The myogenin mRNA expression was the lowest in D20, followed by an obviously increase in siniperca chuatsi, and F1 secondly, while S. scherzeri stable. It showed correlations between muscle growth and gene expression in three mandarin at different growth stages.

关 键 词：鳜鱼 生长 肌纤维 MYOSTATIN MYOD MYOGENIN MRNA表达量 

分 类 号：S917.4[农业科学—水产科学]