二株茶薪菇菌丝多糖深层发酵的培养特征比较与条件控制  

Culture Characteristic Comparison and Condition Control of Fermentation of Two Polysaccharides Agrocybe chaxingu

作　　者：张筱梅[1] 朱维红[1] 贾春凤[1] 

机构地区：[1]河北保定学院生化系,河北保定071000

出　　处：《北方园艺》2014年第23期127-129,共3页Northern Horticulture

基　　金：河北省教育厅计划指导资助项目(Z2011106);河北省自然科学基金资助项目(C2013104055);河北省科技厅科技计划资助项目(12236308)

摘　　要：以2株茶薪菇为试材,研究比较了其多糖深层发酵的培养特征及发酵的控制条件。结果表明：茶薪菇多糖发酵罐深层培养以26℃,pH 6.5,150-250r/min搅拌,0.03-0.05MPa罐压,0.2-0.4m^3/h通风,发酵96h较适宜;菌丝胞内多糖达937-1 080mg/g。2个菌株罐批培养多糖高峰期与摇瓶一致,但产量略高,较固体栽培子实体多糖高1.3-1.5倍,时间缩短12倍。比较发现,菌株U27厚垣孢子数量较多,液体培养效果较好,摇瓶种子菌球小而齐,直径1.0-2.0mm,密度（1.5~2.5）×10^3个/mL,液体深层发酵的菌丝多糖达1 080mg/g,为子实体多糖的1.5倍,是1株良好的多糖深层发酵的生产菌株。Taking two samples of polysaccharides Agrocybe chaxingu as material, the culture characteristic and control condition of fermentation were compared. The results showed that a suitable condition of 26℃,pH 6.5,150--250 r/min stir,0. 03--0. 05 MPa pressure,0. 2--0. 4 m^3/h airflow, and a duration of 96 hours to produce 937--1 080 mg/g of polysaccharide. Tank batch culture shared the same peak period of polysaccharide as that of bottle shaking, but yielded slightly higher. Compared against solid cultivation of fruiting bodies, they yielded 1.3--1.5 times more polysaccharide, and the duration has been shortened by 12-fold. By comparison,the U27 had more chlamydospore,and was more suitable for liquid culture was discovered. By bottle shaking, the fungus balls were small and neat, with diameters between 1. 0 and 2. 0 mm, density between （1.5- 2. 5）× 10^3/mL. The submerged fermentation in liquid yielded up to 1 080 mg/g polysaccharide,which was 50% more than that of fruiting, and was proved to be a healthy choice of polysaccharide submerged fermentation.

关 键 词：茶薪菇 深层发酵 多糖 培养特征 比较 

分 类 号：S646.9[农业科学—蔬菜学]