桦褐孔菌漆酶基因片段的克隆与序列分析  

Cloning and Sequence Analysis of Laccase Gene Fragments from Strains of Inonotus obliquus

作　　者：尹勇刚[1] 隋飞飞[1] 陈艳秋[1] 

机构地区：[1]延边大学长白山生物资源与功能分子教育部重点实验室,延吉133002

出　　处：《吉林农业大学学报》2014年第5期558-563,共6页Journal of Jilin Agricultural University

基　　金：国家自然科学基金项目(31160408)

摘　　要：通过简并引物克隆得到了桦褐孔菌及同属真菌共11个菌株的漆酶基因片段,并对其结构与同源性进行了分析。结果表明:11个目的扩增产物均含有2个内含子,且内含子位置保守,推导的氨基酸序列中均包含有保守的铜离子结合位点;DNA序列与氨基酸序列的相似度都颇高,基于2类序列构建的NJ系统进化树均能将除俄罗斯采集的桦褐孔菌菌株外归为1组,该漆酶基因片段可用于鉴定桦褐孔菌。Laccase gene fragments of 11 strains of Inonotus obliquus and several other species in the same genus were cloned by using degenerate primers, and its structure and homology were also ana？lyzed. The results indicated that two introns, the positions of which were highly conserved, were contained in all 11 PCR-amplified products, and conserved copper？binding sites （ His） were shown in the alignment of amino acid sequences. High similarities were reveled in both DNA and amino acid sequences, and the NJ trees constructed on the basis of the two sequences displayed that all I. obliquus strains expect Chaga were grouped together, suggesting this laccase gene fragment could be applied in species identification of I. obliquus.

关 键 词：桦褐孔菌 漆酶 基因结构 序列分析 

分 类 号：Q785[生物学—分子生物学] Q949.329