Expression and function of natural antisense transcripts in mouse embryonic stem cells  

作　　者：ZHAO Tong WU Zhao Ting WANG Si Yu CHEN Ling Yi 

机构地区：[1]State Key Laboratory of Medicinal Chemical Biology, Collaborative Innovation Center for Biotherapy, 2011 Collaborative Innovation Center of Tianjin for Medical Epigenetics and College of Life Sciences, Nankai University

出　　处：《Science China(Life Sciences)》2014年第12期1183-1190,共8页中国科学（生命科学英文版）

基　　金：supported by the National Natural Science Foundation of China(31271547);the Natural Science Foundation of Tianjin,China(14JYBJC23600);the National Key Basic Research and Development Program of China(2010CB833603);the Program for New Century Excellent Talents(NCET-13-0293);the 111 Project Grant(B08011);the Funds for National Basic Science Personnel Training(J1103503)

摘　　要：Non-coding RNAs(nc RNAs),such as micro RNAs and large intergenic non-coding RNAs,have been shown to play essential roles in regulating pluripotency.Yet,it is not clear the role of natural antisense transcripts(NATs),also belonging to nc RNAs,in embryonic stem cells.However,the role of NATs in embryonic stem cells remains unknown.We further confirmed the expression of the NATs of three key pluripotency genes,Oct4,Nanog and Sox2.Moreover,overexpression of Sox2-NAT reduces the expression of Sox2 protein,and slightly enhances the Sox2 m RNA level.Altogether,our data indicated that like other nc RNAs,NATs might be involved in pluripotency maintenance.Non-coding RNAs（nc RNAs）,such as micro RNAs and large intergenic non-coding RNAs,have been shown to play essential roles in regulating pluripotency.Yet,it is not clear the role of natural antisense transcripts（NATs）,also belonging to nc RNAs,in embryonic stem cells.However,the role of NATs in embryonic stem cells remains unknown.We further confirmed the expression of the NATs of three key pluripotency genes,Oct4,Nanog and Sox2.Moreover,overexpression of Sox2-NAT reduces the expression of Sox2 protein,and slightly enhances the Sox2 m RNA level.Altogether,our data indicated that like other nc RNAs,NATs might be involved in pluripotency maintenance.

关 键 词：natural antisense transcripts embryonic stem cells SOX2 OCT4 PLURIPOTENCY 

分 类 号：Q78[生物学—分子生物学]