细胞外基质蛋白SRPX2对结肠癌SW480细胞生物学行为的影响  

Effect of extracellular matrix protein SRPX2 on the biological behavior of colon cancer cell line SW480

作　　者：周跃[1] 刘揆亮[2] 吴静[2] 

机构地区：[1]兰州大学第一医院消化内科,730000 [2]首都医科大学附属北京世纪坛医院消化内科

出　　处：《中华实验外科杂志》2014年第12期2748-2750,共3页Chinese Journal of Experimental Surgery

基　　金：北京市自然科学基金资助项目（7133239）;北京市卫生系统高层次卫生技术人才培养计划资助项目（2011-RC1）

摘　　要：目的 检测细胞外基质（ECM）SRPX2白在结肠癌组织中的表达,观察上调SRPX2达对结肠癌SW480细胞的影响.方法 采用免疫组织化学方法在结肠癌组织芯片中检测SRPX2表达;采用pCDNA 3.1-SRPX2粒转染SW480细胞,应用噻唑蓝（MTT）法检测24、48、72 hSW480细胞的增殖能力,Transwell侵袭和迁移实验检测转染后SW480细胞的侵袭、迁移能力.结果 免疫组织化学检测结果示SRPX2结肠癌中表达较癌旁组织明显增强（P＜0.01）;MTT检测示48 h及72 h SRPX2染组细胞增殖水平明显增加（P＜0.05）;Transwell法检测,SRPX2染组结肠癌细胞迁移及侵袭能力明显增强（P＜0.01）.结论 SRPX2结肠癌组织中表达增强,并可增强结肠癌细胞的增殖、侵袭及迁移能力,可能起到促癌作用.Objective To investigate the expression of SRPX2protein in cancer tissues,and the effect of SRPX2on the biological behavior of colon cancer cell line SW480.Methods The expressions of SRPX2in colon cancer tissue microarray （TMA） including 31 pairing cancer tissue and adjacent normal tissue were examined with immnuohistochemistry （IHC） method.Previously construvted pCDNA 3.1-SRPX2and pCDNA 3.1 plasmid were transiently transfected into SW480 cells.The proliferation of cells were detected by using MTT assay at 24,48,72 hours after tansfection respectively.Transwell assay was adopted to determine migration and invasion ability of SW480.Results In colon cancer TMA,IHC showed that the expression of SRPX2was significantly higher in colon cancer than in paired noncancerous tissue （P 〈 0.01）.MTT suggested that the proliferation of SW480 cells in the pCDNA 3.1-SRPX2transfected group was signifieantly higher than that in the NC group at respective time of 48,72 hours （P 〈 0.01,P 〈 0.05）,transwell with or without matrigel showed that overexpression of SRPX2significantly increased migratory and invasive numbers of SW480 cells （P 〈 0.01）.Conclusion SRPX2protein is overexpressed in colon cancer and the overexpression of SRPX2could enhance the proliferation,migration and invasion of colon cell line SW480.SRPX2may play an oncogenic role in colon cancer.

关 键 词：SRPX2 SW480 细胞增殖 细胞迁移 侵袭 

分 类 号：R735.35[医药卫生—肿瘤]