多烯紫杉醇对食管癌EC109细胞增殖、凋亡的影响  

Influence of docetaxel on proliferation and apoptosis of human esophageal cancer EC109 cells

作　　者：胡述提[1] 金冰[1] 林涛[1] 

机构地区：[1]河南省南阳市中心医院胸外科,473009

出　　处：《中华实验外科杂志》2014年第12期2761-2763,共3页Chinese Journal of Experimental Surgery

摘　　要：目的 观察多烯紫杉醇对食管癌EC109细胞株增殖、凋亡的影响.方法 采用噻唑蓝（MTT）比色法检测多烯紫杉醇对EC109细胞生长的抑制作用,采用流式细胞仪技术检测多烯紫杉醇对EC109细胞周期和细胞凋亡的影响,Western blot测定多烯紫杉醇对EC109细胞蛋白激酶B（Akt）-1蛋白表达的影响.结果 MTT结果显示多烯紫杉醇作用EC109细胞株后,抑制率随浓度增高而增高（P＜0.05）,流式细胞仪检测结果显示,多烯紫杉醇作用于EC109细胞48 h后,G2/M期细胞均显著增多（P＜0.05）;细胞凋亡检测结果显示,多烯紫杉醇均能明显诱导EC109细胞凋亡（P＜0.05）,多烯紫杉醇10.00 nmol/L组的作用效果最佳,Western blot结果显示多烯紫杉醇明显抑制Akt-1蛋白的表达.结论 多烯紫杉醇对人食管癌EC109细胞的生长有明显的抑制作用,使细胞分裂阻滞于G2/M期,并诱导肿瘤细胞凋亡.Objective To study the influence of docetaxel on proliferation and apoptois of esophageal squamous cell carcinoma EC109 cells in vitro.Methods Methyl thiazol tetrazolium （MTT） assay was used to detect the inhibition effect of docetaxel on the growth of EC109 cells.Flow cytometry was applied to detect the impact of docetaxel on cell cycle and apoptosis of EC109 cells.Western blotting was used to determine the effect of docetaxel on protein kinase B （Akt）-1 protein expression in EC109 cells.Results MTT assay showed that the inhibition rate in the experiment group was increased with the increases of docetaxel concentrations in EC109 cells （P 〈 0.05）.Results of cell cycle detected by flow cytometry showed that the number of cells in G2/M stage was increased notably after treatment of EC109 cells with docetaxel for 48 h （P 〈 0.05）.Tesuhs of apoptosis showed that oridonin could obviously induce the apoptosis of EC109 cells （P 〈0.05）,and the effect was optimal when the oridonin concentration was 10.00 nmol/L.Docetaxel significantly inhibited the expression of Akt-1 protein.Conclusion Docetaxel can significantly inhibite the growth of human esophageal cancer EC109 cells and induce apoptosis and G2/M arrest.

关 键 词：多烯紫杉醇 食管癌 增殖 细胞周期 

分 类 号：R735.1[医药卫生—肿瘤]