HPLC法同时测定补肾通络方中马钱苷、阿魏酸和二苯乙烯苷的含量  

Simultaneous Determination of Loganin,Ferulic Acid and Stibene Glucoside in Bushen Tongluo Fomula by HPLC

作　　者：刘湘丹[1] 黄攀[2] 袁林祥 卢岳华[3] 马铭[2] 

机构地区：[1]湖南中医药大学药学院,长沙410208 [2]湖南师范大学化学化工学院,长沙410081 [3]湖南师范大学医学院,长沙410081

出　　处：《中国药房》2014年第47期4455-4457,共3页China Pharmacy

基　　金：国家中医药管理局中医药重点学科资助项目(No.国中医药发[2009]30号);湖南省高校科研项目(No.12A098);湖南省中药学重点学科建设项目资助(No.湘教通[2011]76号);湖南省大学生研究性学习和创新性实验计划与竞赛项目(No.2013176)

摘　　要：目的：建立同时测定补肾通络方中马钱苷、阿魏酸和二苯乙烯苷含量的方法。方法：采用高效液相色谱法。色谱柱为Hypersil C18（250 mm×4.6 mm,5μm）,流动相为甲醇-0.1%醋酸水溶液（35∶65,V/V）,检测波长为240 nm（马钱苷）、320 nm（阿魏酸和二苯乙烯苷）,柱温为30℃,流速为1.0 ml/min,进样量为10μl。结果：马钱苷、阿魏酸和二苯乙烯苷的质量浓度分别在0.050～2.500（r=0.999 9）、0.005～0.250（r=0.999 9）、0.060～3.000（r=0.999 8）μg/ml范围内与各自峰面积积分值呈良好线性关系;精密度试验的RSD〈2%,稳定性、重复性试验的RSD〈3%;马钱苷、阿魏酸和二苯乙烯苷的平均加样回收率分别为98.31%（RSD=1.92%,n=6）、96.33%（RSD=1.91%,n=6）和100.78%（RSD=1.93%,n=6）。结论：该方法简单、准确,灵敏度高,可用于补肾通络方的质量控制。OBJECTIVE ： To establish a method for simultaneous determination of loganin, ferulic acid and stibene glucoside in Bushen tongluo fomula. METHODS： HPLC method was adopted. The determination was performed on Hypersil C18, （250 mm× 4.6 mm, 5 μm） column with mobile phase consisted of methanol-0.1% acetic acid solution （35：65, V/V） at the flow rate of 1.0 ml/min. The detection temperature was 30 ℃ and sample size was 10 μl. The detection wavelength was set at 240 nm for loganin and 320 nm for ferulic acid and stibene glucoside. RESULTS： The linear range were 0.050-2.500 μg/ml for loganin（r=0.999 9）, 0.005-0.250 μg/ml for ferulic acid （r=0.999 9） and 0.060-3,000 μg/ml for stibene glucoside （r=0.999 8）, respectively. RSD of precision test was lower than 2%, and RSDs of stability and reproducibility tests were lower than 3%. The average recoveries were 98.31% （RSD=1.92% , n=6） , 96.33% （RSD=1.91% , n=6） and 100.78% （RSD=1.93% , n=6） , respectively. CONCLUSIONS： The method is simple, accurate and sensitive, and can be used for the quality control of Bushen tongluo fomula.

关 键 词：补肾通络方 马钱苷 阿魏酸 二苯乙烯苷 高效液相色谱法 含量测定 

分 类 号：R927.2[医药卫生—药学]