肺囊康定B_0发酵培养基的优化研究  

Optimization of fermentation medium for pneumocandin B_0

作　　者：朱春燕 曹艳茹 黄运昌 龙燕 王富强 朱辉 田敏[2] 

机构地区：[1]成都雅途生物技术有限公司,成都610041 [2]四川抗菌素工业研究所,成都610052

出　　处：《中国抗生素杂志》2014年第12期896-900,共5页Chinese Journal of Antibiotics

基　　金：四川省国际科技合作与交流研究计划(编号:2014HH0005)

摘　　要：目的通过优化发酵培养基,提高肺囊康定的B0组分(PB0)产量。方法采用响应面分析法,根据中心组合设计原理及Design Expert 7.0软件分析,基于正交试验结果,对PB0发酵培养基中影响产量的四个主要因子进行配方优化,利用Numerical Optimization功能预测最大响应值并进行验证试验及发酵罐放大试验。结果获得最优发酵配方,摇瓶PB0产量较对照提高了35%,20M3发酵罐PB0产量较对照提高了38%。结论通过响应面分析法优化出发酵培养基,可使PB0的产量大幅提高,并成功地用于工业化生产。Objective To improve the yield of pneumocandin B0 （PB0） by optimizing fermentation medium. Methods By Central Composite Design principles and Design Expert 7.0 analysis software, based on the results of orthogonal test, the method of Response Surface Methodology with 4 factors and 3 levels was adopted on optimizing four main factors which affect the yield of PB0. The maximum response was predicted by Numerical Optimization. The verification test and the scale-up experiment in 20M3 fermenter was carried out. Results Under the optimum condition, the yield of PB0 was respectively 35% and 38% higher than control in shake flask and in 20M3 fermenter. Conclusion By Response Surface Methodology, the yield of PB0 was significantly increased, and successfully using in industrial production.

关 键 词：肺囊康定 YT-B1074-UN-237 响应面分析法 发酵 

分 类 号：R978.15[医药卫生—药品]