Vendela月季产业化快繁体系研究  

Plant Regeneration of Rosa chinensis ‘Vendela'for Scale-up Production via Direct Qrganogenesis

作　　者：李坤峰[1,2] 陈志 陈剑平[1,2] 季杭峰[3] 

机构地区：[1]南京农业大学植保学院,江苏南京210095 [2]浙江省农业科学研究院,浙江杭州310021 [3]杭州鸿雁电器有限公司,浙江杭州310023

出　　处：《核农学报》2014年第10期1790-1797,共8页Journal of Nuclear Agricultural Sciences

基　　金：农业部"引进国际先进农业技术"(2011-G31);地方合作项目"植物组培LED光源的应用和产业化研究"(2012R21B00D01)

摘　　要：本研究以大花月季Vendela为试验材料,利用直接器官发生途径,对影响大花月季组培产业化快繁生产体系的关键环节进行了研究,以期为大花月季种苗组培产业化生产提供技术指导.结果表明：Vendela外植体最佳消毒处理方式是先用洗洁精溶液浸泡30min,75％的酒精处理40s后再用0.1％的氯化汞消毒处理10min;腋芽萌发诱导的最适宜培养基为MS ＋0.5 mg·L-1 6-BA ＋0.05 mg·L-1 NAA＋蔗糖30 g·L-1;不定芽增殖的最适宜培养基为MS＋1.2 mg·L-16-BA ＋0.05 mg·L-1NAA＋30g·L-1蔗糖;继代时每丛2个芽培养后期新生不定芽整齐度好,叶色浓绿,植株健壮,方便不定芽生根诱导材料选择;最适宜的生根培养基为1/2MS ＋0.5 mg·L-1IBA＋30g·L-1蔗糖,且不定根的长度在0.5～1 cm时移栽较容易,苗成活率可达95％以上,整个快繁体系达到组培苗产业化生产的要求.The key steps involved in the establishment of a scale-up production propagation system of Rosa chinensis ＇Vendela＇ via direct organogenesis were discussed in this study. The results showed that the optimum sterilization method was that the explants were washed in detergent solution for 30 min, immersed in 75% ethanol for 40 s and then immersed in the solution of 0. 1% （w/v） mercuric chloride for 10 min; the optimum medium for axillary bud elongation was Murashige-Skoog （MS） medium as basal medium added O. 5 mg· L-1 6 -benzyladenine （6 -BA）, O. 05 mg·L-1 a -naphthaleneacetic acid （NAA） and 30 g· L-1 sucrose; the optimum medium for adventitious buds proliferation was MS medium supplied with 1.2 mg·L-1 6 - benzyladenine （6 - BA）, 0.05 mg· L- 1 a - naphthaleneacetic acid （NAA） and 30 g·L - 1 sucrose ; The best initial number of buds for subculture were 2 buds per cluster, which led to better qualities of adventitious buds than other treatments in this study; The optimum medium for root induction was half concentration of MS medium containing indole - 3 - butyric acid （IBA） O. 5 mg· L-1 and 30 g· L-l sucrose and the survival rate of plantlet in the greenhouse could exceed 95% when the length of root was about 0.5 ~ 0. 1 cm when transferred. All the results showed that the micropropagation system established in this study could be used for scale-up propagation of ＇Vendela＇ via plant tissue culture.

关 键 词：月季 快速繁殖 植物组织培养 器官发生 产业化 

分 类 号：S685.12[农业科学—观赏园艺]