二氯喹啉酸降解菌MC-10的筛选、鉴定及其降解特性  

Screening, identification and characterization of a quinclorac-degrading Arthrobacter sp. MC-10

作　　者：张顺[1,2] 黄国联 许家来 李斌[5] 李宏光 陈德鑫[2] 

机构地区：[1]中国农业科学院研究生院,北京100000 [2]中国农业科学院烟草研究所,山东青岛266001 [3]湖南省烟草公司郴州市公司,湖南郴州423000 [4]山东省烟草研究院,山东济南250101 [5]中国烟草总公司四川省公司,四川成都610000

出　　处：《微生物学报》2015年第1期80-88,共9页Acta Microbiologica Sinica

基　　金：中国烟草总公司资助项目(110200902065);四川省烟草公司资助项目(201302005);山东省烟草公司资助项目(201001);湖南省烟草公司资助项目(12-14Aa03)~~

摘　　要：【目的】为治理稻-烟轮作田块上茬土壤中二氯喹啉酸残留问题,筛选高效降解细菌菌株。【方法】通过富集培养和选择培养,从常年施用二氯喹啉酸的水稻田中筛选可以降解二氯喹啉酸的细菌;对其进行形态学观察、生理生化特征测定和16S r DNA序列系统发育鉴定。【结果】分离的降解菌株MC-10被鉴定为节杆属菌株(Arthrobacter sp.)。菌株MC-10在5%接种量p H 7、28℃时,对初始浓度为20 mg/L二氯喹啉酸7 d可降解90%以上。该降解菌的最佳降解条件为p H 7、30℃,二氯喹啉酸初始浓度在1-100mg/L间均有良好的降解效果;菌株MC-10在土壤中对二氯喹啉酸同样有良好的降解效果,温室内7 d对二氯喹啉酸污染土壤的修复率可达70%。【结论】菌株MC-10在二氯喹啉酸污染土壤和水质治理中具有潜在的应用前景。[ Objective] We screened and isolated bacterial strains from the perennial administration quinclorac paddy fields to degrade quinelorac. [ Methods] Strain MC-10, which can degrade quinclorac efficiently, was screened by enrichment and selective medium. The strain was identified by morphological, physio-biochemical characteristics and 16S rDNA gene sequence analysis. [ Results] MC-10 was identified as Arthrobacter sp. Under the optimal growth conditions with inoculum concentration of 5% , at 28℃, pH 7 for 7 d, MC-10 degraded more than 90% of quinclorac. MC-10 can effectively degrade quinclorac when quinclorac initial concentration at 1 mg/L -100 mg/L. And the strain MC-10 has a good ability to survive in the soil. More than 70% quinclorac in the soil can be degraded efficiently after 7 days of cuhivation. [ Conclusion ] Arthrobacter sp. MC-10 could be a promising microorganism in dealing with quinclorac pollution.

关 键 词：二氯喹啉酸 微生物修复 节杆菌 药害 

分 类 号：X172[环境科学与工程—环境科学]